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目的建立健胃消食片中熊果酸和橙皮苷的含量测定方法。方法采用毛细管区带电泳、高频电导检测,1.2 mmol.L-1三乙胺-HCl(pH10.6)、0.24 mmo.lL-1β-环糊精为运行缓冲液,分离电压12.5 kV,重力进样10 s(高度20 cm)。结果以布洛芬为内标,熊果酸、橙皮苷的线性范围分别为10.55~105.50 g.ml-1(r=0.9992)、11.25~113.50 g.ml-1(r=0.9993);平均回收率分别为97.7%、97.6%。结论所建方法简便、快速、准确可靠,可用于健胃消食片的质量控制。
Objective To establish a method for the determination of ursolic acid and hesperidin in Jianweixiaoshi tablets. Methods Capillary zone electrophoresis and high frequency conductivity detection were used. 1.2 mmol.L-1 triethylamine-HCl (pH10.6) and 0.24 mmo.lL-1β-cyclodextrin were used as running buffers. The separation voltage was 12.5 kV. Injection 10 s (height 20 cm). Results With ibuprofen as internal standard, the linear ranges of ursolic acid and hesperidin were 10.55-105.50 g.ml-1 (r=0.9992) and 11.25-113.50 g.ml-1 (r=0.9993); The recovery rates were 97.7% and 97.6%, respectively. Conclusion The established method is simple, rapid, accurate and reliable, and can be used for quality control of Jianweixiaoshi tablets.