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目的了解玻连蛋白(Vitronectin,VTN)对肝肿瘤细胞株SMMC 7721生长的影响及其对抗3,3'-二吲哚甲烷(3,3'-diindolylmethane,DIM)诱导SMMC 7721凋亡的作用,并初步探讨该过程中所涉及的信号分子。方法采用细胞增殖试剂观察VTN对细胞增殖率的影响,采用激光共聚焦显微镜观察VTN微管蛋白形态的影响,采用流式细胞术和Western Blotting观察VTN对抗植物化学物诱导SMMC 7721细胞凋亡及所涉及的信号分子表达变化。结果 VTN可以剂量依赖的方式促进肿瘤细胞的生长,并可抑制细胞凋亡诱导剂对细胞诱导凋亡的作用。结论体外实验条件下,VTN有助于肝癌细胞株SMMC 7721生长并可保护SMMC7721细胞,使其免予受到凋亡诱导剂的作用,提示VTN在肝癌发生过程中可能具有促进肿瘤细胞生长、抑制化学物诱导凋亡的生物学作用。
Objective To investigate the effect of Vitronectin (VTN) on the growth of hepatoma cell line SMMC 7721 and its effect on the apoptosis of SMMC 7721 induced by 3,3'-diindolylmethane (DIM) And preliminary discussion of the signal molecules involved in the process. Methods The effects of VTN on cell proliferation rate were observed by cell proliferation reagent. The morphological changes of VTN tubulin were observed by laser confocal microscopy. The apoptosis of SMMC 7721 cells induced by phytochemicals was observed by flow cytometry and Western Blotting Changes in signal molecule expression involved. Results VTN could promote the growth of tumor cells in a dose-dependent manner and inhibit the apoptosis-inducing effect of apoptosis inducers on cells. Conclusions VTN can promote the growth of hepatocellular carcinoma cell line SMMC 7721 and protect the SMMC7721 cells from being induced by apoptosis inducing agent in vitro. It suggests that VTN may promote tumor cell growth and inhibit the growth of hepatocellular carcinoma Biological effects of apoptosis induction.