目的：探讨肺表面活性物质（ＰＳ）；与哮喘的关系。方法：①测定肺灌洗液总磷脂、饱和磷脂及总蛋白含量；②观测ＰＳ表面活性；③哮喘发作后给予ＰＳ对肺功能的影响。结果：①哮喘时灌洗液中总磷脂、饱和磷脂无改变；蛋白质显著增加（Ｐ＜００１）；②灌洗液中ＰＳ活性明显降低，表现为最小表面张力增加、恢复系数减小和稳定系数降低；③哮喘时动脉血氧分压、肺顺应性、潮气量等指标明显低于对照组（Ｐ＜００１），给予ＰＳ后１０分钟上述指标开始变化，３０和６０分钟则分别较前显著升高（Ｐ＜００５或００１）。结论：①豚鼠哮喘发作时存在ＰＳ相对不足或功能不良；②给予外源性ＰＳ能缓解哮喘。 Objective: To investigate the relationship between pulmonary surfactant (PS) and asthma. Methods: ①The content of total phospholipids, saturated phospholipids and total protein in lung lavage fluid were measured; ② The surface activity of PS was observed; ③ The effect of PS on pulmonary function after asthma attack was observed. Results: (1) There was no change in total phospholipid and saturated phospholipid in lavage fluid and the protein was significantly increased (P <001) in asthma. The PS activity in lavage fluid was significantly decreased with the increase of minimum surface tension and decrease of recovery coefficient The index of arterial oxygen tension, lung compliance and tidal volume were significantly lower in asthma group than those in control group (P <001). The above indexes began to change 10 minutes after PS administration, while 30 and 60 minutes respectively Significantly higher than before (P <005 or 001). Conclusion: ① guinea pig asthma attack when there is a relative lack of PS or dysfunction; ② exogenous PS can relieve asthma.