论文部分内容阅读
目的 :研究共刺激B7- 1(CD80 )在诱导有效的抗肿瘤免疫反应中所起的作用。方法 :在体外 ,三种肿瘤细胞与同源小鼠脾淋巴细胞混合培养 (MLTCs)后 ,测定淋巴细胞增殖指数 (MTT法 )和特异杀伤活性 (LDH释放改良法 ) ;MTT法测定肿瘤细胞体外增殖能力后 ,将B16 -neo和B16 -mB7- 1细胞分别接种于C57BL/6小鼠皮下后观察成瘤期、荷瘤小鼠存活期以及肿瘤结节生长速度。结果 :与野生型B16细胞和模拟转染的B16细胞 (B16 -neo)比较 ,mB7- 1基因转染的B16细胞 (B16 -mB7- 1)在体外刺激淋巴细胞增殖和诱导CTLs的能力明显增强(P <0 0 5 ) ;尽管两种细胞在体外无明显差异 ,但是与B16 -neo相比 ,B16 -mB7- 1细胞在同源小鼠体内生长速度明显减慢 (P <0 0 5 ) ,成瘤潜伏期和荷瘤小鼠存活期均明显延长 (P <0 0 5 )。结论 :将B7- 1基因导入弱免疫原性的黑色素瘤B16细胞中表达能增强其免疫原性 ,在体内外较有效地诱导CTL介导的抗肿瘤免疫反应。
Objective: To investigate the role of costimulatory B7-1 (CD80) in inducing effective anti-tumor immune responses. METHODS: In vitro, three tumor cells were cultured with homologous mouse splenic lymphocytes (MLTCs), and the lymphocyte proliferation index (MTT assay) and specific killing activity (LDH release improvement assay) were measured; MTT assay was used to determine the tumor cells in vitro. After proliferation, B16-neo and B16-mB7-1 cells were inoculated subcutaneously in C57BL/6 mice, and tumorigenesis, survival of tumor-bearing mice, and growth rate of tumor nodules were observed. RESULTS: Compared to wild-type B16 cells and mock-transfected B16 cells (B16-neo), mB7-1 gene-transfected B16 cells (B16-mB7-1) had significantly increased lymphocyte proliferation and CTLs induction in vitro. (P <0 05); Although there was no significant difference between the two cells in vitro, the growth rate of B16-mB7-1 cells in the homologous mice was significantly slower than that of B16-neo (P <0 05). The latency of tumor formation and survival of tumor-bearing mice were significantly prolonged (P < 0.05). CONCLUSION: The expression of B7-1 gene in weakly immunogenic melanoma B16 cells can enhance its immunogenicity and effectively induce CTL-mediated anti-tumor immune responses in vitro and in vivo.