The water-soluble part(GS) of Ganoderma sinense Zhao,Xu et Zhang was divided into high molecu-lar(GS-H) and low molecular(GS-L) parts by Cellulose Super Filtration,and GS was also fractionated into four frac-tions,GS-1,2,3,and 4 by ethanol precipitation according to their molecular weights.Chemical analysis shows that GS and GS-1,2,3,4 were complexes of polysaccharide and peptide.The fractions with molecular weights over 4000,GS-1,2,3,and GS-H show anti-tumor activities,however,the fractions with molecular weights lower than 4000,GS-4,and GS-L have no anti-tumor activity,indicating that the anti-tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000.Two purified glucopeptides,GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography.Their molecular weights,glycosidic linkages,and configurations were detected by means of IR spectrum,sugar composition analysis,and me-thylation analysis.The polysaccharide parts of GS-6b and GS-7b had glucan backbone consisting of β-1→3 Glc,and side chain containing glucosyl,mannosyl,fucosyl,xylosyl,galactosyl,and glucuronic acid residues attached on 1-2,1-4,1-6 positions of the backbone of GS-6b,or 1-6,1-4 positions of the backbone of GS-7b.The peptide parts in GS-6b and GS-7b were composed of 10 kinds of amino acids,including Asp,Ser,Arg,Gly,Thr,Pro,Ala,Val,Met,and Lys. Xu et Zhang was divided into high molecular-lar (GS-H) and low molecular (GS-L) parts by Cellulose Super Filtration, and GS was also fractionated into four frac -tions, GS-1, 2, 3, and 4 by ethanol precipitation according to their molecular weights. Chemical analysis shows that GS and GS-1,2,3,4 were complexes of polysaccharide and peptide. fractions with molecular weights over 4000, GS-1,2,3 and GS-H show anti-tumor activities, however, the fractions with molecular weights lower than 4000, GS-4, and GS-L have no anti-tumor activity, indicating that the anti -Tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000. Two purified glucopeptides, GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography. Their molecular weights, glycosidic linkages, and configurations were detected by means of IR spectrum, sugar composition analysis, and me-thylation analysis. polysacc haride parts of GS-6b and GS-7b had glucan backbone consisting of β-1 → 3 Glc, and side chain containing glucosyl, mannosyl, fucosyl, xylosyl, galactosyl, and glucuronic acid residues attached on 1-2, 1-4, 1-6 positions of the backbone of GS-6b, or 1-6, 1-4 positions of the backbone of GS-6b and GS-7b are composed of 10 kinds of amino acids, including Asp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Met, and Lys.