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目的建立一种鸡蛋中阿苯达唑(albendazole,ABZ)兽药及其代谢产物阿苯达唑亚砜(albendazole sulfoxide,ABZ-SO)和阿苯达唑砜(albendazole sulfone,ABZ-SO_2)的新监测方法。方法将目前已知的苯并咪唑类药物标准样品直接进质谱分析,考察其二级碎裂质谱行为,找出其共同的特征子离子。鸡蛋样品经乙腈提取浓缩后,经5 mL 20 mmol/L磷酸盐缓冲液(p H 7.0)溶解,再经氧化镍涂层硅胶(SiO_2@Ni O)萃取柱进行固相萃取,最后采用m/z 159特征子离子进行母离子扫描监测阿苯达唑药物及其代谢产物。结果采用本方法监测到喂食阿苯达唑药物的母鸡产的鸡蛋中的阿苯达唑残留及其代谢产物,ABZ,ABZ-SO和ABZ-SO_2的检出限分别为2.2、5和3 ng/g。结论该方法快速、准确,适合食品中阿苯达唑及其代谢产物的分析监测。
Objective To establish a new animal model of albendazole (ABZ) veterinary drug and its metabolites albendazole sulfoxide (ABZ-SO) and albendazole sulfone (ABZ-SO_2) in eggs. Monitoring method. Methods The standard samples of benzimidazole currently known were directly analyzed by mass spectrometry, and the secondary fragmentation mass spectrometry behavior was investigated to find out their common characteristic ion. The egg sample was extracted with acetonitrile, concentrated, then dissolved in 5 mL 20 mmol / L phosphate buffer (p H 7.0), and then solid-phase extraction was performed on a NiO-coated silica gel column (SiO 2 @ Ni O) z 159 Signature ions Detect albendazole drugs and their metabolites using parent ion scan. Results The detection limits of albendazole and its metabolites in hen eggs fed albendazole were detected by this method. The detection limits of ABZ, ABZ-SO and ABZ-SO 2 were 2.2, 5 and 3 ng / g. Conclusion The method is rapid, accurate and suitable for the analysis and monitoring of albendazole and its metabolites in food.