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目的:应用近红外光谱技术建立快速测定知母中芒果苷含量的方法。方法:以HPLC所测样品中芒果苷的含量为参考值,将近红外光谱与知母中芒果苷的含量进行关联,对光谱预处理方法和建模区间进行了考察,采用偏最小二乘法(PLS)建立定量分析模型。结果:通过比较最终确定以SNV+SG+Second Derivative对光谱进行预处理,结合软件的波长区间优化功能,确定4 139.58~5 068.56 cm-1为最佳波段。结果 100份知母样品所建立的芒果苷的定量分析模型内部交叉验证决定系数(R2)校正均方根偏差(RMSEC)、预测均方根偏差(RMSEP)分别为0.963 61,0.061 6,0.088 1。结论:该方法准确、快速、简便,建立的定量分析模型,能够对知母中芒果苷的含量进行快速测定。
Objective: To establish a rapid method for the determination of mangiferin in Zhimu by near-infrared spectroscopy. Methods: The content of mangiferin in the samples measured by HPLC was taken as a reference value, and the correlation between near-infrared spectra and mangiferin contents in A. annua was studied. The spectral pretreatment method and modeling interval were investigated. Partial least squares (PLS) Establish a quantitative analysis model. Results: The spectra were preprocessed by SNV + SG + Second Derivative by comparison and finally the optimal wavelength band of 4 139.58 ~ 5 068.56 cm-1 was determined with the optimization function of wavelength range of software. Results The root mean square deviation (RMSEC) of cross validation coefficient (R2) of mangiferin in the quantitative analysis model of mangiferin was established based on the 100 samples of mangiferin. The root mean square deviation of prediction (RMSEP) was 0.963 61,0.061 6,0.088 1 . Conclusion: This method is accurate, rapid, simple, and established quantitative analysis model, which can quickly determine the content of mangiferin in.