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目的探讨Krppel样因子4(krppel-like factor 4,KLF4)对人胃癌SGC-7901细胞株生长增殖的影响。方法以人胃癌SGC-7901细胞株为研究对象,脂质体介导转染pcDNA3.1IE-KLF4重组质粒并建立KLF4稳定过表达细胞株,以未转染KLF4基因和转染空质粒载体的胃癌细胞株为对照。用RT-PCR和免疫细胞化学法检测KLF4 mRNA和蛋白的表达,流式细胞术检测细胞周期及增值指数。结果 RT-PCR和免疫细胞化学法检测示转染组细胞与未转染组和空载体组相比较,KLF4 mRNA和蛋白均明显表达(P<0.05)。流式细胞术检测显示转染组G1期细胞增多[(65.08±3.43)%,P<0.05],而S期和G2/M期细胞减少[(30.39±1.80)%、(4.53±1.66)%,P<0.05],增殖指数亦降低[(34.92±3.42)%,P<0.05]。结论 KLF4对SGC-7901细胞株体外生长增殖具有抑制作用。
Objective To investigate the effect of Kröppel-like factor 4 (KLF4) on the growth and proliferation of human gastric cancer cell line SGC-7901. Methods Human gastric cancer cell line SGC-7901 was used as the research object. The recombinant plasmid pcDNA3.1IE-KLF4 was transfected by Lipofectamine 2000 and KLF4 stable overexpression cell line was established. The untransfected KLF4 gene and gastric cancer cells transfected with empty plasmid Cell lines as controls. The expression of KLF4 mRNA and protein was detected by RT-PCR and immunocytochemistry. The cell cycle and proliferation index were detected by flow cytometry. Results The mRNA and protein of KLF4 in transfected cells were significantly higher than those in untransfected and empty vector groups (P <0.05) by RT-PCR and immunocytochemistry. Flow cytometry showed that the number of cells in the G1 phase of the transfected group was significantly increased (65.08 ± 3.43%, P <0.05), while the percentage of cells in the S phase and the G2 / M phase was decreased by 30.39 ± 1.80% and 4.53 ± 1.66% , P <0.05], and the proliferation index also decreased [(34.92 ± 3.42)%, P <0.05]. Conclusion KLF4 can inhibit the growth and proliferation of SGC-7901 cells in vitro.