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目的探讨IL-10-819C/T基因多态性与急性心肌梗死(AMI)的相关性。方法抽取325例AMI患者(A组)和197例正常对照人群(B组)的外周静脉血,提取DNA,回收PCR产物,进一步测序鉴定IL-10-819C/T基因多态性。结果与B组相比,A组吸烟和合并糖尿病患者比例增加,血清TC、TG和LDL水平升高(P<0.01或P<0.05)。IL-10-819C/T位点在A组和B组中均存在CC、CT和TT基因型,但两组间基因型频率和等位基因频率比较无统计学差异(P>0.05);A组3种基因型行多个相关危险因素Logistic分析与AMI发病仍无相关性(P>0.05)。结论 IL-10-819C/T基因多态性与苏南地区汉族人群AMI发病可能无关。
Objective To investigate the association between IL-10-819C / T polymorphism and acute myocardial infarction (AMI). Methods Peripheral venous blood was collected from 325 patients with AMI (group A) and 197 normal controls (group B). The DNA was extracted and the PCR products were recovered. The polymorphisms of IL-10-819C / T gene were identified by sequencing. Results Compared with group B, the proportion of smokers and patients with diabetes mellitus in group A increased and the levels of serum TC, TG and LDL increased (P <0.01 or P <0.05). The genotype frequencies of CC, CT and TT in IL-10-819C / T locus were found in both groups A and B, but there was no significant difference between the two groups in genotype frequency and allele frequency (P> 0.05). A There was no correlation between Logistic analysis of multiple risk factors and incidence of AMI in three genotypes (P> 0.05). Conclusion The IL-10-819C / T polymorphism may not be related to the incidence of AMI in Han population of southern Jiangsu province.