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目的 探讨颈部交感神经对三叉神经轴浆运输的影响。方法 选用 48只Wistar大白鼠 ,右侧为实验侧 ,左侧对照侧 ,用微量注射器在双侧眶下区对称的部位注射 30 %辣根过氧化酶 (horseradishperoxidase,HRP) 5μl,同时在实验侧C5颈椎横突处注射 0 .5 %布比卡因和确炎舒松A各半的混悬液 0 .4ml,在对侧注射 0 .9%的生理盐水 0 .4ml作为对照。动物分别存活 4、6、8、10、12、14h后 ,经升主动脉灌注杀死。取双侧颈上交感神经节和三叉神经节 ,冰冻切片后利用TMB法进行显色反应 ,观察切片内HRP标记阳性细胞的情况 ,应用图像分析系统对双侧颈上交感神经节和三叉神经节内阳性细胞进行分类和计数 ,并分析双侧阳性细胞的平均面积和平均光密度。结果 实验侧三叉神经节最早出现标记细胞的时间是 6h ,对照侧是 8h ,实验侧HRP轴浆运输的速度是 (5 .5 0± 0 .95 )mm/h ,对照侧是 (3 .99± 0 .81)mm/h(P <0 .0 1)。实验侧三叉神经标记细胞平均面积和及平均面积和与光密度乘积均较对照侧大 (P <0 .0 1) ,双侧交感神经节均于 8h后出现标记细胞 ,但对照侧HRP标记细胞平均面积和及平均光密度较实验侧大 (P <0 .0 1)。结论 颈部交感神经可影响三叉神经轴浆运输速度 ,行颈部局封可减缓颈部交感神经轴浆运输 ,而促进三叉神经轴浆?
Objective To investigate the effect of cervical sympathetic nerve on axonal transport of trigeminal nerve. Methods Forty-eight Wistar rats were selected. On the right side of the experimental group, the left side of the control group was injected with 5μl of horseradish peroxydase (HRP) 30μl at the symmetrical position of the bilateral infraorbital zone with a microinjector. At the same time, C5 transverse cervical vertebra 0.5% injection of bupivacaine and Yan Shu Song A half suspension of 0.4 ml, 0. 9% contralateral 0.9 ml saline 0.4 ml as a control. Animals were survived 4,6,8,10,12,14 h, after ascending aorta perfusion to kill. Both sides of the cervical sympathetic ganglia and trigeminal ganglion were harvested, and frozen sections were used for color reaction by TMB method. HRP-labeled positive cells in the sections were observed. Image analysis system was used to examine the relationship between bilateral cervical sympathetic ganglia and trigeminal ganglion Positive cells were sorted and counted, and the average area and average optical density of bilateral positive cells were analyzed. Results The time of first labeled cells in the experimental trigeminal ganglion was 6h and the control side was 8h. The velocity of axial paracentesis was (5.50 ± 0.95) mm / h on the experimental side and (3.99 ± 0.81) mm / h (P <0.01). On the experimental side, the average area and the average area of the labeled trigeminal neurons and the product of the optical density and density were significantly larger than those of the control side (P <0.01). The labeled cells appeared in the bilateral sympathetic ganglia after 8 hours. However, The average area and the average optical density were larger than those on the experimental side (P <0.01). Conclusions Cervical sympathetic nerves can affect axonal transport velocity of the trigeminal nerve. The administration of cervical collar can slow the axonal transport of the sympathetic nerve in the neck and promote axonal pulp of the trigeminal nerve.