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美国白蛾核型多角体病毒（ Ｈｃ Ｎ Ｐ Ｖ） 编码的几丁质酶 Ａ（ Ｃｈｉｔｉｎａｓｅ Ａ， Ｃｈｉ Ａ） 基因定位在 Ｈｉｎｄ Ⅲ－ ３５ｋｂ 片段上。 Ｄ Ｎ Ａ 测序表明，半胱氨酸蛋白酶（ Ｃ Ｐ） 基因的５＇上游反向存在 Ｃｈｉ Ａ基因， Ｃｈｉ Ａ 基因的 Ｏ Ｒ Ｆ 为１ ６６２ 个核苷酸，编码５５３ 个氨基酸，５＇、３＇非编码区分别具有 Ｔ Ａ Ａ Ｇ 基序和 Ａ Ａ Ｔ Ａ Ａ Ａｐｏｌｙ（ Ａ＋ ） 信号序列。同源性分析表明， Ｈｃ Ｎ Ｐ Ｖ 的 Ｃｈｉ Ａ 与苜蓿丫纹夜蛾核型多角体病毒、家蚕核型多角体病毒的 Ｃｈｉ Ａ ，在 Ｄ Ｎ Ａ 水平上的同源性分别为７５３ ％ 、７５７ ％ ，在氨基酸水平上的同源性分别为７５０ ％ 、７８７ ％ ，与来自灵菌的 Ｃｈｉ Ａ 比较，一致性氨基酸达５１ ％ ，推测杆状病毒几丁质酶基因与细菌几丁质酶基因可能具有共同的祖先。
Chitinase A (Chi A) gene encoded by HcNPV was mapped on Hind Ⅲ-3.5 5kb fragment. D N A sequencing showed that the Chi A gene was located upstream of the 5 ’upstream of the cysteine protease (C P) gene and the ORF of Chi A gene was 1 662 nucleotides encoding a 553 amino acid, 5’ The 3 ’noncoding region has the T A A G motif and the A A T A A Apoly (A +) signal sequence, respectively. Homology analysis showed that the homology between Chi A of Hc N P V and Chi A of B. saxicophila and B. bombycis nucleopolyhedrovirus was 753 % And 757%, respectively. The homologies at the amino acid level were 750% and 787%, respectively. Compared with Chi A from the inbred strain, the identity of amino acids was 51%. It was presumed that the baculovirus chitin The enzyme gene and the bacterial chitinase gene may have a common ancestor.