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目的 研究 β连环素 (β cat)在吸烟小鼠不同阶段气道上皮中表达的变化 ,探讨不同功能状态的β cat在气道上皮损伤修复中的作用。 方法 采用HE染色光镜观察研究小鼠吸烟 5周组(S5w组 ) ,吸烟 10周组 (S10w组 ) ,吸烟 15周组 (S15w组 )气道上皮的形态学变化 ;应用原位杂交及免疫组化技术研究气道上皮 β catmRNA、β cat、原癌基因 (c Src)、酪氨酸磷酸化蛋白 (phosphorytyrsine,p Tyr)的表达。结果 (1)随着吸烟时间的延长气道上皮形态学上呈损伤 (S5w)、修复 (S10w)、过度增殖 (S15w)变化。 (2 )S5w、S10w、S15w组 β catmRNA及蛋白表达水平均较对照组低 (对照组、S5w、S10w、S15w组 β cat染色平均吸光度值分别为 0 2 0 4± 0 0 12、0 0 97± 0 0 0 7、0 16 0± 0 0 0 3、0 10 7±0 0 0 8;β catmRNA染色平均吸光度值分别为 0 2 2 3± 0 0 0 8、0 12 3± 0 0 11、0 173± 0 0 0 8、0 116± 0 0 0 4,P <0 0 1)。但S10w组 β catmRNA及蛋白表达水平较S5w、S15w组高。S15w组中尚可见 β cat核内表达。 (3)正常气道上皮细胞c Src、p Tyr表达极弱 ,和S5w、S10w、S15w组相比差异有显著性 (对照组、S5w、S10w、S15w组c Src染色平均吸光度值分别为 0 0 48± 0 0 15、0 12 7± 0 0 18?
Objective To investigate the expression of β cat in the airway epithelium of smoking mice and to explore the role of β cat in the repair of airway epithelium injury. Methods HE staining was used to observe the morphological changes of airway epithelium in 5-week smoking group (S5w group), 10-week smoking group (S10w group) and 15-week smoking group (S15w group) To study the expression of β cat mRNA, β cat, c Src and p Tyr in airway epithelium by histochemical technique. Results (1) The morphological changes of airway epithelium (S5w), repair (S10w) and hyperproliferation (S15w) were observed with the prolongation of smoking time. (2) The β cat mRNA and protein expression levels in S5w, S10w and S15w groups were lower than those in control group (The average absorbance values of β cat in control group, S5w, S10w and S15w group were 0 2 0 4 ± 0 0 12,0 0 97 ± 0 0 0 7,0 16 0 ± 0 0 0 3,0 10 7 ± 0 0 0 8; The average absorbance values of β cat mRNA were 0 2 2 3 ± 0 0 0 8,0 12 3 ± 0 0 11, 0 173 ± 0 0 0 8,0 116 ± 0 0 0 4, P <0 0 1). However, the β cat mRNA and protein expression in S10w group were higher than those in S5w and S15w group. S15w group is still visible in the β cat nuclear expression. (3) The expressions of c-Src and p-Tyr in normal airway epithelial cells were very weak, which were significantly different from that in S5w, S10w and S15w groups (the mean absorbance values of c-Src in control group, 48 ± 0 0 15,0 12 7 ± 0 0 18?