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为提高食品中致病菌快速检测的准确性,设计了一种基于三磷酸腺苷(ATP,adenosine 5′-triphosphate)生物发光反应动力学数据拟合以及光学校准的食品致病菌检测系统。采用与ATP生物发光波长相同的LED模拟生物发光,对检测样品的光吸收及散射进行校准,从而减小食品颜色及浊度的干扰。采用光电倍增管(PMT)连续记录ATP生物发光强度数据,基于生物发光反应动力学方程自动对发光强度数据拟合,减小系统噪声并提高仪表的测量范围。研究了基于LED的光学校准算法,基于ATP生物发光动力学研究了数据拟合算法,进而设计实现了致病菌检测系统。对金黄色葡萄球菌样品的检测结果表明,本文系统与传统培养计数方法相关性达到0.98,检测致病菌范围为102~108 CFU/mL,对单一样品测量的精密度为5.05%。本文系统设计为生物发光在食品安全检测领域的实际应用提供了新的思路,具有重要实际意义。
In order to improve the accuracy of rapid detection of pathogenic bacteria in food, a food pathogen detection system based on the bioluminescence reaction kinetic data of ATP (adenosine 5’-triphosphate) and optical calibration was designed. Using the same LED as the bioluminescence of ATP to simulate bioluminescence, the light absorption and scattering of the test sample are calibrated to reduce the interference of food color and turbidity. The data of ATP bioluminescence was recorded continuously by photomultiplier tube (PMT), and the luminescence intensity data was automatically fitted based on the bioluminescence reaction kinetic equation to reduce the system noise and improve the measuring range of the instrument. The optical calibration algorithm based on LED was studied, the data fitting algorithm was studied based on ATP bioluminescence kinetics, and then the pathogen detection system was designed and realized. The test results of the Staphylococcus aureus samples showed that the correlation between the system and traditional culture counting method was 0.98, the range of pathogenic bacteria was 102-108 CFU / mL, and the precision of the single sample was 5.05%. The system design of this article provides a new idea for the practical application of bioluminescence in the field of food safety detection and has important practical significance.