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目的研究藳本内酯和丁烯酉夫内酯与大鼠主动脉平滑肌细胞的亲合活性,及其对主动脉平滑肌细胞增殖的抑制作用。方法采用大鼠主动脉细胞膜色谱模型观察本内酯和丁烯酉夫内酯的保留特性;培养并分离纯化大鼠主动脉平滑肌细胞,采用bFGF诱导平滑肌细胞增殖,以MTT比色法检测本内酯和丁烯酉夫内酯对平滑肌细胞增殖的抑制作用。结果本内酯和丁烯酉夫内酯与大鼠主动脉平滑肌细胞膜有亲和性,其保留行为与钙离子受体拮抗剂维拉帕米相似;本内酯和丁烯酉夫内酯不会引起正常大鼠主动脉平滑肌细胞增殖,但能明显抑制bFGF诱导的大鼠主动脉平滑肌细胞的增殖。其有效浓度分别为5.5和11.1μmol.L-1(P<0.05)。结论本内酯和丁烯酉夫内酯与大鼠主动脉平滑肌细胞具有亲和力,能抑制血管平滑肌细胞的异常增殖。
Objective To study the affinity of ligustilide and butene-y-lactone to rat aortic smooth muscle cells and their inhibitory effects on the proliferation of aortic smooth muscle cells. Methods The rat aorta plasma membrane chromatographic model was used to observe the retention properties of the lactone and butene-fuf lactone. The aortic smooth muscle cells of rats were isolated and purified. The proliferation of smooth muscle cells was induced by bFGF. MTT assay Inhibition of Smooth Muscle Cell Proliferation by Ester and Butyryllin Lactone. Results Intravenous and butene-γ-butyrolactone had affinity with rat aortic smooth muscle cell membrane, and its retention behavior was similar to that of the calcium receptor antagonist verapamil. Can cause normal rat aortic smooth muscle cell proliferation, but can significantly inhibit bFGF-induced rat aortic smooth muscle cell proliferation. The effective concentrations were 5.5 and 11.1 μmol.L-1, respectively (P <0.05). Conclusion Intra-ligustilide and butene-y-lactone have affinity with rat aortic smooth muscle cells and inhibit the abnormal proliferation of vascular smooth muscle cells.