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采用不同剂量的60 Co γ射线诱变处理杂交当代干种子,以 MW14 和改良 MS 为基本培养基;从MF1 或MF2 代中选择优良单株为供体进行花药培养。针对辐射诱变剂量、低温预处理供体幼穗、变温诱导培养和低温预处理愈伤组织与花培出愈率、绿苗分化率的关系以及越夏控苗和花粉植株移栽等问题进行了研究,以提高获得遗传稳定的可育性加倍单体—纯合二倍体花粉植株的频率,通过大田系谱法可快速选育出新的小麦突变体品种( 系) 及育种材料。这种小麦单倍体辐射诱变育种途径不仅保留了辐射诱变育种固有的特点,同时可将育种周期缩短3 ～4 个世代,提高了小麦辐射诱变育种的效率。
Different generations of 60 Co γ-rays were used to mutagenize the hybridized contemporary dry seeds with MW14 and modified MS as the basic medium. The best single plant from MF1 or MF2 was selected as the donor for anther culture. The aim of this study was to investigate the effects of irradiation dose, low-temperature pre-treatment donor panicles, temperature-induced induction and low temperature pretreatment on the rate of callus induction, the rate of green shoot differentiation and the transplanting of seedlings and pollen plants To improve the frequency of genetically stable, fertile double haploid-pollen diploid pollen plants, and to rapidly breed new wheat mutant varieties and breeding materials by field pedigree method. This wheat haploid radiation mutation breeding not only retained the inherent characteristics of radiation mutation breeding, breeding cycle can be shortened 3 to 4 generations and improve the efficiency of wheat radiation mutation breeding.