论文部分内容阅读
目的:探讨γδT细胞在过敏性支气管哮喘发病机制中的作用。方法:来自门诊的40例粉尘螨和屋尘螨点刺阳性、部分控制过敏性支气管哮喘患者随机分为常规治疗组(A组)和吸入减敏组(B组),每组各20例。所有患者接受相同的基础治疗,常规治疗组雾化吸入生理盐水5mL、吸入减敏组雾化吸入特异性变应原,每周2次,持续6个月。治疗前后用流式细胞术(FCM)检测外周血及诱导痰中γδT细胞占淋巴细胞百分比;IL-4+、IFN-γ+γδT细胞占淋巴细胞百分比。结果:治疗后B组外周血、诱导痰中γδT细胞占淋巴细胞百分比低于治疗前;B组外周血和诱导痰中IFN-γ+γδT细胞占淋巴细胞百分比增高。结论:特异性变应原雾化吸入减敏疗法有效防治哮喘可能与γδT细胞有关;γδT细胞可能参与哮喘发病机制。
Objective: To investigate the role of γδT cells in the pathogenesis of allergic bronchial asthma. Methods: Forty dust mites and house dust mites from the clinic were stabbed positive. Some patients with allergic bronchial asthma were randomly divided into routine treatment group (group A) and inhalation desensitization group (group B), 20 cases in each group. All patients received the same basic treatment, conventional treatment group inhalation of saline 5mL inhalation desensitization group inhalation of specific allergens, 2 times a week for 6 months. Before and after treatment, the percentages of γδT cells in peripheral blood and induced sputum in lymphocytes were detected by flow cytometry (FCM); the percentage of lymphocytes of IL-4 +, IFN-γ + γδT cells. Results: After treatment, the percentages of γδT cells in peripheral blood and induced sputum were lower than those before treatment; the percentage of IFN-γ + γδT cells in peripheral blood of induced sputum and the percentage of lymphocytes in induced sputum increased. Conclusion: Specific allergen inhalation desensitization therapy for the prevention and treatment of asthma may be related to γδT cells; γδT cells may be involved in the pathogenesis of asthma.