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百日咳全细胞菌苗经液体培养、酸沉淀和56℃30分钟脱毒制成.无细胞制剂是酸沉淀后经两次离心(15000g×15分钟)及磷酸盐浸出提取.以小鼠体重变化判定毒性试验结果,用百日咳杆菌作脑内攻击检测两种制剂对小鼠的保护力.将两种制剂以不同剂量接种小鼠后5天测定组胺致敏活性,计算24小时内小鼠死亡数,按Reed等方法计算结果.结果表明,6μg无细胞制剂即可产生93.75～100%的保护作用;而600μg的全细胞菌苗只能使75.00～81.25%的小鼠存活.在本实验所用的剂量范围内,两种制剂均未使小鼠的体重发生变化.两种制剂产生的组胺致敏活性呈剂量反应关系,但全细胞菌苗
The B. pertussis whole-cell vaccine was prepared by liquid culture, acid precipitation and detoxification at 56 ℃ for 30 minutes.The cell-free preparation was acid-precipitated and centrifuged twice (15000g × 15min) and phosphate leaching Toxicity test results showed that B. pertussis was used as an intracerebral challenge to test the protective effect of the two preparations on mice.The histamine sensitization activity was measured 5 days after the two formulations were inoculated at different doses to calculate the number of mice dead in 24 hours , And the results were calculated according to the method of Reed et al .. The results showed that 6μg of cell-free preparation could produce a protection effect of 93.75-100%, while 600μg of whole cell vaccine could only make 75.00-81.25% of mice survive.In the experiment, In the dose range, the body weight of the two formulations did not change the body weight.The histamine sensitization activity of the two formulations showed a dose-response relationship, but the whole cell vaccine