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目的：通过免疫组化方法研究增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）在肾盂输尿管移行细胞癌中表达的临床意义。方法：１９７６年８月—１９９１年８月收治存档的上尿路移行细胞癌组织蜡块３９例行免疫组化分析。采用Ｓ－Ｐ法，一抗为ＰＣ１０，膀胱移行细胞癌切片做阳性对照，肾盂积水切片做阴性对照。每张切片计数五个视野，求出ＰＣＮＡ指数。ＰＣＮＡ指数与临床分期、病理分级间比较用方差分析，多变量生存分析用Ｃｏｘ模型。结果：ＰＣＮＡ阳性染色为肿瘤细胞核染成棕黄色，分布不均，与对照片对比鲜明。染色阳性细胞数目、着色深度与分期分级关系密切。ＰＣＮＡ指数截断点定为２５％。≤２５％与＞２６％两组术后五年生存率差异显著。Ｃｏｘ模型表明ＰＣＮＡ指数是非常重要的一项预后指标。结论：ＰＣＮＡ是一个有临床意义的细胞增殖标记物。ＰＣＮＡ提供的细胞动力学信息反映了肿瘤的生物学行为。ＰＣＮＡ指数是一个客观的定量指标。ＰＣＮＡ指数与分期分级关系密切，可作为分期分级的一种辅助方法。
Objective: To investigate the clinical significance of proliferating cell nuclear antigen (PCNA) expression in renal pelvis and ureter transitional cell carcinoma by immunohistochemistry. Methods: Immunohistochemical analysis of 39 paraffin-embedded tissues of upper urinary tract transitional cell carcinoma from August 1976 to August 1991 was performed. Using S-P method, primary antibody PC10, bladder transitional cell carcinoma slice to do a positive control, hydronephrosis section as a negative control. Count five fields per slice and find the PCNA index. PCNA index and clinical stage, histological grade comparison analysis of variance, multivariate survival analysis with Cox model. Results: PCNA positive staining of tumor cells stained brown, uneven distribution, with the contrast of the picture. The number of stained positive cells, the depth of stained grading and staging closely related. PCNA index truncation point was set at 25%. The difference of 5-year survival rate between two groups of 25% and> 26% was significant. Cox model shows that PCNA index is a very important prognostic indicator. Conclusion: PCNA is a clinically significant cell proliferation marker. The cellular dynamics provided by PCNA reflect the biological behavior of the tumor. PCNA index is an objective quantitative index. The PCNA index is closely related to staging classification, and can be used as an auxiliary method for staging classification.