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本试验主要对常规的柑桔茎尖嫁接脱毒技术进行了改进,将传统的“T”法茎尖嫁接改为顶端横切面直接嫁接.结果表明,改进的顶接法操作简便,迅速,成活率较高,达51.04%,较倒“T”法成活率32.97%净增18.04%,且新法嫁接苗的生长速度明显快于倒“T”法.试验还得出不同的培养基质对茎尖嫁接苗的成活也有差异,采用MS液体培养基试管内培养成活率为35.7%,而在营养土钵内培养成活率达49.5%.
In this experiment, we improved the conventional technology of grafting and detoxification of citrus shoots, and changed the traditional “T” method of stem tip grafting into the top cross-section directly grafting.The results showed that the improved top-joining method was simple, rapid and survival The rate of growth was 51.04%, which was 32.04% higher than that of “T” method, and the net growth rate was 18.04%. The growth rate of the new grafted seedlings was obviously faster than that of “T” method. The survival rate of grafted seedlings is also different. The survival rate of in vitro culture using MS liquid culture medium is 35.7%, while the survival rate in culture soil bowl is 49.5%.