目的　阐明中国胰腺癌患者K -ras基因 12密码子突变方式。方法　采用 3′碱基特异性引物PCR技术分析 30例已证明存在突变的胰腺癌DNA标本。结果　检测到 4种突变方式即GAT、GTT、CGT和GCT ,比例分别为 5 2 .9%、32 .4%、8.8%和 5 .9%。结论　中国胰腺癌K -ras基因 12密码子存在 4种突变方式 ,因地区不同可能存在差异 Objective To clarify the mutation of K-ras gene codon 12 in Chinese patients with pancreatic cancer. Methods 30 cases of pancreatic cancer DNA samples that had been proved to be mutated were analyzed by 3 ’base - specific PCR. Results Four kinds of mutations were detected, namely GAT, GTT, CGT and GCT, with the proportions of 52.9%, 32.4%, 8.8% and 5.9% respectively. Conclusion There are four mutations in the 12-codon of K-ras gene in Chinese pancreatic cancer, which may be different according to different regions