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目的:建立五味渣驯丸中羟基红花黄色素A、没食子酸、马兜铃酸A的含量测定方法。方法:采用HPLC对制剂中主要成分羟基红花黄色素A、没食子酸、马兜铃酸A进行定量分析。CAPCELL PAK C18色谱柱(4.6 mm×250 mm,5μm),甲醇-0.4%磷酸(30∶70)、甲醇-0.2%磷酸(7∶93)、甲醇-0.05%冰醋酸(79∶21)为流动相,流速1.0 mL.min-1,检测波长为403,273,390 nm。结果:羟基红花黄色素A、没食子酸、马兜铃酸A的线性范围分别为0.116～1.740μg(r=0.999 97),0.067～0.673μg(r=0.999 95),0.024～0.247μg(r=0.999 99);平均回收率分别为98.62%(RSD 2.15%),100.01%(RSD 2.03%),96.83%(RSD 1.74%)。结论:本方法简便、结果准确可靠,可有效控制制剂质量。
Objective: To establish a method for the determination of hydroxysafflor yellow A, gallic acid and aristolochic acid A in Wuwei Residue Tailor. Methods: The main components in the preparation of hydroxysafflor yellow A, gallic acid, aristolochic acid A quantitative analysis. CAPCELL PAK C18 column (4.6 mm × 250 mm, 5 μm), methanol -0.4% phosphoric acid 30:70, methanol -0.2% phosphoric acid 7:93, methanol -0.05% glacial acetic acid 79:21 Phase, the flow rate of 1.0 mL.min-1, detection wavelength of 403,273,390 nm. Results: The linear ranges of hydroxysafflor yellow A, gallic acid and aristolochic acid A were 0.116 ~ 1.740μg (r = 0.99997), 0.067 ~ 0.673μg (r = 0.99995), 0.024 ~ 0.247μg = 0.999 99). The average recoveries were 98.62% (RSD 2.15%), 100.01% (RSD 2.03%) and 96.83% (RSD 1.74%), respectively. Conclusion: The method is simple, the results are accurate and reliable, which can effectively control the quality of preparation.