荞麦为蓼科一年生草本植物,其果实中含有大量的酚性化合物,本次探讨了它的抗氧化作用。 方法:体外试验:按照Az-ma等的方法检测O_2~T、DPPH,以ESR检测·OH。体内试验:对Wistar雄性大鼠(5周龄)分别经口给予荞麦提取物100、200mg/(kg·d),共给予20d。其后结扎肾动静脉45min,再灌注120min,采集血液并摘出肾。 Buckwheat is an annual herb of Polygonaceae, and its fruit contains a large number of phenolic compounds. The antioxidation effect of this buckwheat is discussed. Methods: In vitro test: O 2 -T, DPPH were detected according to the method of Az-ma et al., and OH was detected by ESR. In vivo test: Wistar male rats (5 weeks old) were orally given 100, 200 mg/(kg·d) of buckwheat extract for a total of 20 days. After that, the renal arteriovenous vein was ligated for 45 min and reperfusion for 120 min. Blood was collected and the kidneys were removed.