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目的研究胶体物质羟乙基淀粉（ＨＥＳ）及低流量灌注技术（ＬＦＰ）对兔心肺联合保存的作用。方法选用健康家兔５０只，２只用于正常心肺组织的超微结构观察，４８只随机均分为晶体液（ＣＳ）组、ＨＥＳ组及ＬＦＰ组，每组８对供、受体。用Ｓｔ．Ｔｈｏｍａｓ液及改良Ｅｕｒｏ－Ｃｏｌｌｉｎｓ液灌注ＣＳ组心、肺，含５％ＨＥＳ的Ｓｔ．Ｔｈｏｍａｓ液及改良Ｅｕｒｏ－Ｃｏｌｌｉｎｓ液灌注ＨＥＳ组及ＬＦＰ组心、肺，取心与左肺整体４℃保存７ｈ，保存期间ＬＦＰ组经主、肺动脉持续低流量（０．２５ｍｌ／ｇ心肺重量／ｈ）灌注４℃相应保存液。移植后每３０ｍｉｎ测定供体心左室收缩压（ＬＶｓｐ）、左室舒张末压（ＬＶｅｄｐ）、右房压（ＲＡＰ）及供体肺气道峰压（Ｐａｗ），取动脉血测氧分压（ＰａＯ２），实验结束时记录异丙肾上腺素用量，计算心肺重量增值，测定心肺组织丙二醛（ＭＤＡ）含量，取心肺组织观察超微结构。结果ＣＳ组心肺重量增值高于ＨＥＳ组（Ｐ＜０．０１），ＨＥＳ组与ＬＦＰ组无明显差异。ＣＳ组ＬＶｓｐ均低于ＨＥＳ组（Ｐ＜０．０５或＜０．０１），３０，６０ｍｉｎ时ＬＦＰ组高于ＨＥＳ组（Ｐ＜０．０５）。ＣＳ组ＬＶｅｄｐ均高于ＨＥＳ组（Ｐ＜０．０５或Ｐ＜０．０１）?
Objective To study the effect of hydroxyethyl starch (HES) and low-flow perfusion (LFP) on the cardiopulmonary syndromic preservation in rabbits. Methods Fifty healthy rabbits were selected and used for observing the ultrastructure of normal cardiopulmonary tissues. Forty-eight rabbits were randomly divided into two groups: crystalloid (CS) group, HES group and LFP group. With St. Thomas fluid and modified Euro-Collins fluid perfusion group CS heart, lung, 5% HES containing St. The hearts, lungs and corpus striatum in the LEF group and the LEF group were stored at 4 ℃ for 7h. The LFP group received continuous low pulmonary flow (0.25ml / g heart lung weight / h ) Perfusion 4 ℃ corresponding preservation solution. The left ventricular systolic pressure (LVsp), left ventricular diastolic pressure (LVedp), right atrial pressure (RAP) and donor pulmonary airway pressure (Paw) were measured every 30min after transplantation. (PaO2). At the end of the experiment, the amount of isoproterenol was recorded, the value of cardiopulmonary weight was calculated, the content of malondialdehyde (MDA) in heart and lung tissue was measured, and the lung tissue was taken to observe the ultrastructure. Results The cardiopulmonary weight gain in CS group was higher than that in HES group (P <0.01). There was no significant difference between HES group and LFP group. LVsp in CS group was lower than that in HES group (P <0.05 or <0.01), and LFP group was higher than HES group at 30 and 60min (P <0.05). LVedp in CS group was higher than that in HES group (P <0.05 or P <0.01).