目的 :观察清热解毒、活血化瘀、养阴增液 3种温病治法制剂对内毒素血症大鼠生存率的影响。方法 :将健康 Wistar大鼠随机分为正常对照组、模型对照组及清热解毒组、活血化瘀组和养阴增液组。模型对照组和3种中药干预组大鼠腹腔注射内毒素和 D氨基半乳糖以建立内毒素血症模型 ;记录 2 4小时内各组大鼠死亡数。各组存活大鼠中随机选取 10只检测其血液流变学、肝酶谱及细胞因子的变化。结果 :模型对照组大鼠生存率达 5 5 %,清热解毒组、活血化瘀组和养阴增液组大鼠生存率明显升高 (分别为 90 %、95 %和 95 %)。模型对照组大鼠肿瘤坏死因子 α(TNFα)、白介素 1(IL 1)及天冬氨酸转氨酶 (AST)、丙氨酸转氨酶 (AL T)、碱性磷酸酶 (AL P)明显高于正常对照组 (P均 <0 .0 5 ) ;而各中药干预组上述诸指标均显著低于模型对照组(P<0 .0 5或 P<0 .0 1) ,部分指标乃至接近正常水平。模型对照组和 3种中药干预组间血液流变学各项指标均无明显差异 (P均 >0 .0 5 )。结论 :3种温病治法制剂——清热解毒、活血化瘀和养阴增液注射液均可显著提高内毒素血症大鼠生存率 ,拮抗炎性细胞因子 ,减轻肝细胞损伤 ,对内毒素血症大鼠有一定的保护作用。 Objective: To observe the effects of Qingrejiedu, Huoxuehuayu, Yangyin increase liquid on the survival rate of rats with endotoxemia. Methods: Healthy Wistar rats were randomly divided into normal control group, model control group, Qingrejiedu group, Huoxuehuayu group and Yangyinchang group. Rats in the model control group and the three Chinese medicine intervention groups were injected intraperitoneally with endotoxin and D-galactosamine to establish an endotoxemia model; the number of deaths in each group within 24 hours was recorded. Ten of the surviving rats in each group were randomly selected to examine the changes of hemorheology, liver enzymes, and cytokines. Results: The survival rate of the model control group rats was up to 55%. The survival rate of Qingrejiedu group, Huoxue Huayu group and Yangyin increase fluid group was significantly higher (90%, 95%, and 95%, respectively). Model control rats were significantly higher than normal in tumor necrosis factor alpha (TNF alpha), interleukin 1 (IL 1) and aspartate aminotransferase (AST), alanine aminotransferase (AL T), alkaline phosphatase (AL P) In the control group (P <0.05), all of the above indicators in the Chinese medicine intervention group were significantly lower than those in the model control group (P <0.05 or P <0.01), and some indicators were even close to normal. There were no significant differences in blood rheology between the model control group and the three traditional Chinese medicine intervention groups (all P > 0.05). Conclusion: Three kinds of mild disease treatment preparations - Qingrejiedu, Huoxuehuayu and Yangyin Zengye injection can significantly improve the survival rate of rats with endotoxemia, antagonize inflammatory cytokines, reduce liver cell damage, and Toxemia rats have a protective effect.