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以食蟹猴疟原虫猕猴动物模型,用间接荧光抗体试验法(IFAT)观察疟疾感染过程中特异性IgM抗体的变化,共观察无复燃或复发的短原虫血症期(15天内,5只猴)、长原虫血症期(26～37天,2只猴)及出现复燃(5只猴)3种类型的疟疾感染。疟疾IgM抗体初次出现于子孢子感染后13～16天或阳性血感染后3～9天,抗体滴度高峰出现于子孢子感染后17～25天或阳性血感染后12～26天。尔后抗体逐渐降低,抗体消失时间在原虫血症短者为感染后2个月,在原虫血症长者约3个月,复燃出现在感染终止后约1个月。结果表明,疟疾特异性IgM抗体的出现与存留,是新近疟疾感染的一个佐证。
The animal model of cynomolgous macaques macaque was used to observe the changes of specific IgM antibodies in the process of malaria infection by indirect fluorescent antibody test (IFAT), and to observe the parasitemia without recurrence or relapse (5 in 15 days Macaques), protracted serogroups (26-37 days, 2 monkeys) and relapse (5 monkeys). The malaria IgM antibody first appeared in 13 to 16 days after sporozoite infection or 3 to 9 days after positive blood infection. The highest antibody titer appeared in 17 to 25 days after sporozoite infection or 12 to 26 days after positive blood infection. After the antibody gradually decreased, the disappearance of antibody in the protracted blood short period of 2 months after infection, in the elderly parasitemia about 3 months, resurgence occurred in about 1 month after termination of infection. The results show that the emergence and persistence of malaria-specific IgM antibodies is a corroborating evidence of recent malaria infection.