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目的了解实施天然林保护后迭部县莱姆病在人群中的感染现状,自然疫源地的变迁及宿主的莱姆病感染情况。方法人群血清伯氏疏螺旋体抗体的检测采用免疫荧光法,以聚合酶链反应(PCR)检测伯氏疏螺旋体DNA。结果迭部县境内5个林场及7个乡镇采集居民血清522份,莱姆病抗体阳性57份,阳性率10.92%。捕鼠6种69只,平均捕获率15.33%;其中北社鼠25只,占捕获总数的36.23%,褐家鼠18只,占26.09%,大林姬鼠15只,占21.74%,小林姬鼠9只,占13.04%,小家鼠和黑线姬鼠各1只,各占1.45%。对62份鼠脾脏、66份肾脏,用PCR检测伯氏疏螺旋体核酸,结果阳性鼠脾6份,肾5份,鼠携带莱姆病病原阳性率为15.15%(10/66);其中北社鼠6只,褐家鼠2只,大林姬鼠及小林姬鼠各1只。用BSK培养基从采集的鼠肾、膀胱中分离培养出3株伯氏疏螺旋体。结论迭部县广泛存在莱姆病疫源地,人群感染率较高。
Objective To understand the current situation of Lyme disease in Dieiben County after natural forest protection, the change of natural foci and the host Lyme disease infection. Methods Detection of Borrelia burgdorferi antibodies in human population By immunofluorescence, DNA of Borrelia burgdorferi was detected by polymerase chain reaction (PCR). Results In Duding County, 5 forest farms and 7 townships collected 522 serum samples from residents and 57 patients were positive for Lyme disease. The positive rate was 10.92%. There were 6 species of traps in 69 species, with an average capture rate of 15.33%. Among them, 25 were North rats, accounting for 36.23% of the total, 18 of them were brown rats, accounting for 26.09%, 15 of them were Apodemus, accounting for 21.74% 9, accounting for 13.04%, each of 1 Mus musculus and Apodemus agrarius, each accounting for 1.45%. Sixty-two mouse spleens and 66 kidneys were detected by PCR. Borrelia burgdorferi nucleic acid was detected by PCR. The results showed that there were 6 positive spleens and 5 kidneys. The positive rate of pathogenicity of Lyme disease in mice was 15.15% (10/66) 6 rats, 2 Rattus norvegicus, 1 large Apodemus and 1 small Apodemus. Three strains of Borrelia burgdorferi were isolated from the collected rat kidneys and bladder using BSK medium. Conclusion There are many sources of Lyme disease in Diebu County, with a high infection rate.