论文部分内容阅读
目的：了解新疆两城市丙型肝炎病毒感染者中存在哪些基因型，以便为今后防治ＨＣＶ感染提供依据。方法：用ＲＴ－ＰＣＲ技术，选择ＨＣＶＮＳ－５区设计的分型引物（１ａ、１ｂ、２ａ和２ｂ）进行基因分型。结果：我们检测了１２８例（乌鲁木齐市９７例，克拉玛依市３１例）已证实为丙型肝炎病毒感染者的血清，其中基因分型检测出的９８例中可分为１ａ型１４例（１４．３％）、１ｂ型５８例（５９．３％）、２ａ型１１例（１１．８％）、２ｂ型１０例（１０．３％），混合感染５例（５．２％）（１ａ＋１ｂ型４例，１ｂ＋２ａ型１例）。在乌鲁木齐市和克拉玛依市的ＨＣＶ感染者中１ａ型分别为１１／７３例和３／２５例，１ｂ型分别为４６／７３例和１２／２５例。结论：表明新疆至少存在ＨＣＶ１ａ、１ｂ、２ａ和２ｂ基因型，但以１ｂ基因型感染为主。比较乌鲁木齐市和克拉玛依市两城市的ＨＣＶ基因型分布显示１ａ型和１ｂ型分布有明显差别（χ２＝３６，Ｐ＜０．００１）。另３０例用本方法未能检出分型，提示可能还有其它基因型存在
Objective: To know which genotypes exist in hepatitis C virus in both cities in Xinjiang in order to provide the basis for future prevention and treatment of HCV infection. Methods: RT-PCR was used to select the genotyping primers designed by HCV NS-5 region (1a, 1b, 2a and 2b). Results: We detected 128 cases (97 cases in Urumqi and 31 cases in Karamay) which have been confirmed as hepatitis C virus sera. Of the 98 cases detected, 14 cases were classified as 1a (14 cases). 3 cases), 1b (58%), 1b (11.8%), 2b (10.3%) and 5 (5.2% 4 cases, 1b + 2a type in 1 case). Among patients with HCV infection in Urumqi and Karamay, type 1a were 11/73 and 3/25 respectively, while type 1b was 46/73 and 12/25 respectively. Conclusion: The genotypes HCV1a, 1b, 2a and 2b at least exist in Xinjiang, but the predominant genotype 1b infection. Comparison of HCV genotype distribution between Urumqi and Karamay showed that the distribution of type 1a and 1b were significantly different (χ2 = 36, P <0.001). The other 30 cases failed to detect the typing of this method, suggesting that there may be other genotypes