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目的:探讨乳腺癌组织中EG-1和Her-2的表达及其与乳腺癌临床特征、预后的相关性。方法:采用RT-PCR技术检测EG-1在72例乳腺癌及18例良性乳腺组织中的表达,应用免疫组化技术检测分析Her-2的表达。结果:乳腺癌组织EG-1 mRNA表达(71%)显著高于良性乳腺组织(24%),χ2=17.604,P<0.01。淋巴结转移数目越多,EG-1 mRNA阳性率越高,P<0.05。51例EG-1 mRNA阳性患者中,33例(64.7%)发生复发转移;而21例EG-1mRNA阴性患者中,仅5例(23.8%)发生复发转移,差异有统计学意义,χ2=9.982,P<0.05。单因素生存分析表明,EG-1表达不同的患者间总生存时间(OS)和无病生存时间(DFS)差异均有统计学意义,P<0.05。Cox模型多因素回归分析显示,淋巴结转移数、EG-1 mRNA、Her-2蛋白是预测总生存率和无复发生存率的独立预后因素。结论:EG-1基因在乳腺癌组织中异常高表达,且与淋巴结转移相关,是乳腺癌潜在的预后标志。
Objective: To investigate the expression of EG-1 and Her-2 in breast cancer and its correlation with clinical features and prognosis of breast cancer. Methods: The expression of EG-1 in 72 cases of breast cancer and 18 cases of benign breast tissue was detected by RT-PCR. The expression of Her-2 was detected by immunohistochemistry. Results: The expression of EG-1 mRNA in breast cancer tissue was significantly higher than that in benign breast tissue (71%) (χ2 = 17.604, P <0.01). The higher the number of lymph node metastasis, the higher the positive rate of EG-1 mRNA was (P <0.05). Of the 51 EG-1 mRNA positive patients, 33 (64.7%) had recurrence and metastasis. Of the 21 EG-1 mRNA negative patients Five cases (23.8%) had recurrence and metastasis, the difference was statistically significant, χ2 = 9.982, P <0.05. Univariate survival analysis showed that the difference of total survival time (OS) and disease-free survival time (DFS) among patients with different EG-1 expression was statistically significant (P <0.05). Multivariate regression analysis of Cox model showed that lymph node metastasis, EG-1 mRNA and Her-2 protein were independent predictors of overall and recurrence-free survival. Conclusion: The overexpression of EG-1 in breast cancer tissues is associated with lymph node metastasis and is a potential prognostic marker for breast cancer.