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为了寻求在胚胎发育过程中调控脊神经节(SG)神经元-脊髓背角靶细胞特异感觉通路形成的因素,利用植块联合悬滴培养技术比较研究了鸡胚脊髓背、腹角组织促SG神经突起生长作用的差异.16只Hamburger35期Leghorn鸡胚,10只用于植块联合培养.分别切取鸡胚脊髓L_1节段的左侧背角、右侧腹角组织植块,并与同—脊髓节段的同侧SG进行植块联合悬滴培养.另外6只鸡胚用于单独培养L_1脊髓节段SG作为参照.培养液为Eagle’s MEM,补加1/3小牛血清、葡萄糖5g/L.脊髓背角组织与SG联合培养组(D-G组)、腹角组织与SG联合培养组(V-G组)以及单独SG培养组(G组)分别培养存活10个、9个、11个.于培养24小时、60小时观察测量各个SG神经突起的平均长度.SG神经突起的平均长度是沿SG周缘8个方向上所测神经突起长度的均值.实验结果;培养24小时,各组SG的神经突起平均长度分别为D-G组184.94±12.95(μm,X±SE,下同)、V-G组107.90±16.23、G组133,87±9.81,培养60小时,D-G组558.67±54.70、V-G组392.83-27.17、G组486.92±33.22.统计学分析揭示,从培养24小时到60小时,各组SG神经突起均有明显增长.同一观测时间内,D-G组神经突起平均长度显著大于V-G组者.提示,鸡胚脊髓背、腹角组织对SG神经突起生长作用有明显差异,背角组织能促进SG神经突起?
In order to find out the factors that regulate the formation of specific sensory pathways in the spinal ganglion (SG) neurons and spinal dorsal horn during embryonic development, we used grafting and hanging drop culture techniques to study the effects of SG and SG The difference of growth effect among the sixteen Leghorn hamsters of Hamburger stage35 and the ten of them were used for the co-culture of the explants.The left dorsal horn of the L_1 segment and the right ventral horn of the chick embryo were cut out and compared with the same spinal cord Sixteen chick embryos were used to culture L_1 spinal cord segment SG as a reference.The culture medium was Eagle’s MEM, supplemented with 1/3 calf serum, glucose 5g / L (DG group), combination of SG and SG group, and SG group (G group), 10, 9 and 11 survived respectively 24 hours and 60 hours observed the average length of each SG neurites.The average length of SG neurites is the average length of the neurites measured in 8 directions along the peripheral edge of the SG.Experimental results; cultured 24 hours, SG neurites The average length of DG group was 184.94 ± 12.95 (μm, X ± SE , The same below), VG group 107.90 ± 16.23, G group 133.87 ± 9.81, cultured for 60 hours, DG group 558.67 ± 54.70, VG group 392.83-27.17, G group 486.92 ± 33.22 .Statistical analysis revealed that from the culture for 24 hours At 60 hours, the number of SG neurites in each group increased significantly, and the mean length of neurites in DG group was significantly greater than that in VG group in the same observation time, suggesting that the growth of SG neurite in the dorsoventral and ventral horn of chick embryo was significantly different , Dorsal horn can promote SG neurite out?