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目的:研究白藜芦醇(Res)对体外培养的小鼠大脑皮层神经元线粒体形成的作用。方法:培养神经元分为3组:空白对照组、白藜芦醇组(Res组)、二甲基亚砜组(DMSO组)。处理7d后检测培养神经元ATP含量和线粒体DNA拷贝数,采用Real timePCR方法检测线粒体形成相关分子Tfam、NRF-1、NRF-2和PGC-1α的表达情况。结果:Res处理能够明显增加体外培养小鼠神经元ATP含量,Res组ATP水平明显高于DMSO组(P<0.01);Res组mtDNA拷贝数明显增加(P<0.001),而DMSO组与空白对照组组间mtDNA拷贝数无明显差异。此外,Res明显上调了线粒体形成相关分子Tfam、NRF-1、NRF-2和PGC-1α的基因表达(P<0.001)。结论:Res处理可能具有通过影响mtDNA拷贝数及线粒体形成相关基因的表达,促进小鼠神经元线粒体形成的潜能。
AIM: To investigate the effect of resveratrol on mitochondria formation in cultured mouse cerebral cortical neurons. Methods: Neurons were cultured in 3 groups: blank control group, resveratrol group (DMSO group) and dimethyl sulfoxide group (DMSO group). The ATP content and the copy number of mitochondrial DNA in cultured neurons were detected after 7 days of treatment. The expression of mitochondrial related molecules Tfam, NRF-1, NRF-2 and PGC-1α were detected by Real time PCR. Results: Res treatment significantly increased ATP content in neurons cultured in vitro, and the level of ATP in Res group was significantly higher than that in DMSO group (P <0.01). The copy number of mtDNA in Res group was significantly increased (P <0.001) There was no significant difference in copy number of mtDNA between groups. In addition, Res significantly up-regulated gene expression of mitochondrial-associated molecules Tfam, NRF-1, NRF-2 and PGC-1α (P <0.001). Conclusion: Res treatment may have the potential to promote mitochondria formation in mouse neurons by affecting the expression of mtDNA copy number and mitochondrial formation-related genes.