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目的通过二血管阻断加低血压法建立大鼠急性全脑缺血再灌注模型,探讨在脑缺血再灌注情况下DADLE对于心肺肾的保护作用。方法将50只SD大鼠随机分为5组:假手术组(Sham)、模型组(I/R)、DADLE处理组(根据不同剂量可分为2 mg/kg[A]、3 mg/kg[B]、5 mg/kg[C])。采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型。手术后立即取心肺肾,常规HE染色观察其形态学改变。结果 I/R组心肌细胞间有出血现象,心肌细胞有轻度萎缩,少量炎性细胞浸润;肺脏表现为肺泡间隔增宽,毛细血管扩张充血,肺胞腔内及血管周围中性粒细胞浸润,气管壁部分上皮脱落,肺胞腔及气管腔均有浆液渗出,肺大泡形成越来越多;肾脏表现为肾小球与肾间质有少量充血,肾小球毛细血管未见明显扩张。DADLE处理组心肌出血减少并逐渐被吸收(P<0.05);肺脏充血减轻,中性粒细胞浸润有所减少(P<0.05);肾脏可见肾小球无明显充血肿胀,间质亦无明显瘀血。DADLE处理组组间差异无统计学意义(P>0.05)。结论改良的二血管阻断加低血压法建立大鼠全脑缺血再灌注模型对心肺均有不同程度的损伤,但对肾脏影响较小,并且DADLE对全脑缺血再灌注大鼠心肺损伤有一定的保护作用,但与剂量无明显相关。
OBJECTIVE: To establish a rat model of acute global cerebral ischemia-reperfusion by occlusion of blood vessels and hypotension, and to explore the protective effect of DADLE on cardiopulmonary, renal and renal function in cerebral ischemia-reperfusion rats. Methods Fifty Sprague-Dawley rats were randomly divided into 5 groups: sham group, model group (I / R) and DADLE group (2 mg / kg [A], 3 mg / kg [B], 5 mg / kg [C]). A model of global cerebral ischemia-reperfusion was established by modified two-vessel occlusion plus hypotension. Immediately after surgery, take heart, lung and kidney, and observe the morphological changes by conventional HE staining. Results There were bleeding between myocardial cells in I / R group, slight atrophy of myocardial cells and infiltration of a small amount of inflammatory cells. The lungs showed widened alveolar septum, telangiectasia and hyperemia, infiltration of neutrophil in the lung cavity and perivascular cells , Part of the tracheal wall epithelial shedding, lung cavity and tracheal cavity have effusion, bulla formation more and more; kidney showed a small amount of glomerular and renal interstitium hyperemia, glomerular capillary no obvious expansion. Myocardial hemorrhage was decreased and gradually absorbed in DADLE group (P <0.05); lung congestion was alleviated and neutrophil infiltration was decreased (P <0.05); there was no obvious congestion and swelling in glomerulus and no stasis in interstitial blood. There was no significant difference between DADLE treatment groups (P> 0.05). Conclusion The established model of global cerebral ischemia-reperfusion in rats with bilateral cerebral ischemia-reperfusion has different degrees of injury to heart and lungs, but it has little effect on the kidneys. DADLE can inhibit the injury of heart and lung in rats after global cerebral ischemia-reperfusion Have a certain protective effect, but no significant correlation with the dose.