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灯盏乙素是灯盏花的主要药用成分,研究灯盏乙素合成的相关基因对培育高品质的灯盏花品种有重要意义。本研究通过转录组测序和RT-PCR获得灯盏花黄酮6-羟化酶(flavone 6-hydroxylase,F6H)和7-O-葡糖醛酸转移酶(flavonoid 7-O glucuronosyltransferases,F7GAT)基因c DNA全长,分别命名为EbF6H1、EbF6H2和EbF7GAT。生物信息学软件分析表明灯盏花F6H1 c DNA全长1 478 bp,编码492个氨基酸残基;F6H2 c DNA全长1 524 bp,编码507个氨基酸残基;F7GAT c DNA全长1 311 bp,编码436个氨基酸残基。荧光定量PCR表明F6H1主要在根中表达;F6H2在叶片中表达量最高,其次是根和茎;F7GAT在叶中的表达量显著高于其它器官。HPLC分析表明,叶片中灯盏乙素含量最高,其次是茎和花,在根中未检测到。根中总绿原酸的含量最高,其次是叶,显著高于茎和花。本研究有助于进一步探明基因功能和活性成分积累的关系。
Scutellarin is the main medicinal composition of Erigeron Breviscapine to study the synthesis of scutellar genes related to cultivate high-quality Erigeron breed is important. In this study, we obtained the cDNAs of flavone 6-hydroxylase (F6H) and 7-O-glucuronosyltransferases (F7GAT) genes by transcriptome sequencing and RT-PCR Full-length, were named EbF6H1, EbF6H2 and EbF7GAT. Bioinformatics software analysis showed that the F6H1 c DNA was 1 478 bp in length and encoded 492 amino acid residues. F6H2 c DNA was 1 524 bp in length and encoded 507 amino acid residues. F7GAT cDNA was 1 311 bp in length encoding 436 amino acid residues. Fluorescence quantitative PCR showed that F6H1 was mainly expressed in roots; F6H2 was highest in leaves, followed by roots and stems; F7GAT in leaves was significantly higher than that in other organs. HPLC analysis showed that the content of scutellarin in leaves was highest, followed by stems and flowers, but not detected in roots. Root chlorogenic acid content of the highest, followed by leaves, significantly higher than the stems and flowers. This study helps to further elucidate the relationship between gene function and accumulation of active ingredients.