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本文报道应用亲合色谱分离机制对各种环糊精(CDs)进行的专一性定量法。BioGelP-6经胺化得苯甲酰化的凝胶,使CDs能与淀粉部分水解物及线性麦芽低聚糖分离,样品无需任何特殊制备即可用于分析。苯甲酰化Bio-Gel的制备 Bio-Gel P-625 g置于内有500 ml乙二胺(工业用)的园底烧瓶(附有回流冷凝管及磁力揽拌器)中,于110～115℃下(油浴)不断搅拌4小时后冷却、过滤,然后依次用水、0.2M氯化钠-0.001 M盐酸、水、10%乙醇溶液及水充分洗涤,得每克抽干的湿凝胶含0.57 mmol氨基(电位滴定法)。苯甲酸1g溶于30 ml温水中,用1M氢
In this paper, a specific quantitative method for the determination of various cyclodextrins (CDs) using affinity chromatography separation mechanism was reported. BioGelP-6 is aminated to a benzoylated gel, which separates CDs from partial starch hydrolyzates and linear malto-oligosaccharides. Samples can be used for analysis without any special preparation. Preparation of benzoylated Bio-Gel Bio-Gel P-625 g was placed in a round bottom flask (with reflux condenser and magnetic stirrer) containing 500 ml of ethylenediamine (for industrial use) Stirring was continued for 4 hours at 115 ° C (oil bath), then cooled, filtered and washed thoroughly with water, 0.2M sodium chloride - 0.001 M hydrochloric acid, water, 10% ethanol solution and water to give per gram of dry wet gel 0.57 mmol amino (potentiometric titration). 1 g of benzoic acid was dissolved in 30 ml of warm water and treated with 1 M hydrogen