目的:去除口服头孢菌素类抗生素的抑菌性,建立控制菌微生物限度的检查方法。方法:采用头孢菌素酶,有效地水解头孢菌素β-内酰胺的酰胺键,使其失去抗菌活性。结果:头孢菌素酶能有效地去除头孢菌素类抗生素的抑菌活性,但头孢菌素类抗生素品种不同,所需头孢菌素酶的浓度也不同。结论:该方法操作简便,结果准确可靠,可用于口服头孢菌素类胶囊剂控制菌的检查。 OBJECTIVE: To remove the antibacterial activity of oral cephalosporin antibiotics and to establish a method to check the limits of microorganisms in control bacteria. Methods: Cephalosporins were used to hydrolyze the amide bond of cephalosporin β-lactam effectively to lose antibacterial activity. Results: Cephalosporinase can effectively remove the antibacterial activity of cephalosporin antibiotics, but different cephalosporin antibiotics, the required concentration of cephalosporins are also different. Conclusion: The method is simple, accurate and reliable, and can be used for the control of oral cephalosporin capsules.