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采用沙培方式研究了营养液NO3-浓度对草莓幼苗生长和抗氧化酶系统的影响.结果表明:不同NO3-浓度(16 mmol·L-1,CK;48 mmol·L-1,T1;96 mmol·L-1,T2;144mmol·L-1,T3)处理15 d后,草莓幼苗株高、叶面积、干物质积累量和根冠比均比对照显著降低,其中T1、T2、T3的株高和功能叶叶面积分别比对照降低16.1%、36.8%、43.9%和19.7%、34.3%、47.5%.随NO3-浓度增加,各处理脯氨酸、可溶性蛋白、丙二醛(MDA)含量和电解质渗漏率呈先升高后降低趋势,但均高于对照;除叶片中过氧化氢酶(CAT)活性逐渐降低外,叶片和根系中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性及根系中的CAT活性均呈先升高后降低趋势,其中T3叶片和根系中的SOD活性及根系中的APX活性低于对照.表明NO3-胁迫下,细胞内溶物质增加,部分抗氧化酶活性升高但高浓度下降低,生长势减弱,NO3-浓度为48 mmol·L-1即表现出抑制草莓生长的作用.
The effects of NO3- concentration on the growth and antioxidant enzyme system of strawberry seedlings were studied using sand culture method. The results showed that the NO3- concentration of 16 mmol·L-1, CK; 48 mmol·L-1, T1; 96 The leaf height, leaf area, dry matter accumulation and root / shoot ratio of strawberry seedling were significantly decreased compared with the control after 15 days of treatment, at the same time of T3, mmol·L-1, T2, 144mmol·L-1, T3) The plant height and functional leaf area decreased by 16.1%, 36.8%, 43.9% and 19.7%, 34.3% and 47.5%, respectively. Compared with the control, proline, soluble protein, malondialdehyde (MDA) Content and electrolyte leakage rate increased firstly and then decreased, but both were higher than those of the control. Except for the activity of catalase (CAT) gradually decreased, the activity of superoxide dismutase (SOD) in leaves and roots The activities of POD, APX and CAT in roots were increased first and then decreased. The activity of SOD and the activity of APX in roots were lower than those of the control. The results showed that under NO3-stress, the content of intracellular solutes increased, the activity of some antioxidant enzymes increased but decreased at high concentration, and the growth potential decreased. The NO3- concentration of 48 mmol·L-1 Inhibition of growth of strawberry.