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目的:研究中国HIV感染长期不进展者(Long-term nonprogressors,LTNP)相关免疫指标的变化。方法:采集284例未经抗HIV治疗的LTNP、典型进展的HIV感染者和AIDS病人及130例HIV抗体阴性健康人的抗凝全血,应用流式细胞仪分析技术对CD8+T淋巴细胞、NK细胞及DC细胞进行测定。结果:LTNPCD8+T淋巴细胞绝对值[(1104.51±511.81)个/μl]高于AIDS病人[(678.40±295.39)个/μl,P<0.05]。LTNPNK细胞的绝对值[(377.59±289.23)个/μl]显著高于HIV感染者[(292.49±445.87)个/μl]和AIDS病人[(153.62±110.36)个/μl,P<0.05]。LTNPCD123+DCs细胞绝对值[(6.76±3.74)个/μl]高于HIV感染者[(5.30±3.16)个/μl]和AIDS病人[(3.32±2.09)个/μl,P<0.05]。LTNPCD11c+DCs细胞绝对值[(21.73±11.92)个/μl]高于HIV感染者[(14.51±9.53)个/μl]和AIDS病人[(7.27±3.74)个/μl,P<0.05]。结论:LTNPCD8+T淋巴细胞、NK细胞和DC细胞高于典型进展的HIV感染者和AIDS病人,是延缓疾病进程的重要因素。
Objective: To study the changes of immune indicators related to long-term nonprogressors (LTNP) in China. Methods: A total of 284 cases of LTNP without anti-HIV treatment, typical HIV infected patients and AIDS patients and 130 anticoagulant whole blood of HIV-negative healthy people were collected. Flow cytometry was used to detect CD8 + T lymphocytes, NK cells and DC cells were measured. Results: The absolute value of LTNPCD8 + T lymphocytes [(1104.51 ± 511.81) / μl] was higher than that of AIDS patients [(678.40 ± 295.39) / μl, P <0.05). The absolute value of LTNPNK cells [(377.59 ± 289.23) / μl] was significantly higher than that of HIV-infected patients [(292.49 ± 445.87) / μl] and AIDS patients [(153.62 ± 110.36) / μl, P <0.05]. The absolute value of LTNPCD123 + DCs cells [(6.76 ± 3.74) / μl] was higher than that of HIV infected patients [(5.30 ± 3.16) / μl] and AIDS patients [(3.32 ± 2.09) / μl, P <0.05). The absolute value of LTNPCD11c + DCs cells (21.73 ± 11.92) / μl was higher than that of HIV infected patients (14.51 ± 9.53) / μl and AIDS patients (7.27 ± 3.74) / μl, P <0.05. CONCLUSIONS: LTNPCD8 + T lymphocytes, NK cells, and DC cells are higher than those of typical HIV infected and AIDS patients and are important factors in the progression of the disease.