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目的调查辽宁省部分地区虫媒病毒的种类。方法 2009年8月,在辽宁省鞍山市、朝阳市、葫芦岛市和锦州北镇市采集蚊虫标本。蚊虫经分组研磨后接种C6/36和BHK-21细胞,连续3代观察细胞病变情况;对细胞病变阳性的分离物,进行抗原性检测及分子生物学鉴定。结果在辽宁省上述4个城市共采集蚊虫3600只,主要为中华按蚊(47.2%)、淡色库蚊(26.4%)、刺扰伊蚊(18.1%)及三带喙库蚊(6.9%)等。蚊虫标本分72组进行研磨,通过组织细胞培养法,共获得2株阳性分离物,经抗原性检测及核酸序列测定证实为Ⅰ型流行性乙型脑炎病毒。结论 2009年,从辽宁省鞍山地区的淡色库蚊及朝阳地区的刺扰伊蚊标本中分离到Ⅰ型流行性乙型脑炎病毒。
Objective To investigate the types of arboviruses in some areas of Liaoning Province. Methods In August 2009, mosquitoes were collected from Anshan City, Chaoyang City, Huludao City and Beizhen City, Jinzhou City, Liaoning Province. The mosquitoes were ground in groups and inoculated with C6 / 36 and BHK-21 cells for 3 consecutive passages to observe the cytopathic effect. The cytopathic positive isolates were tested for antigenicity and molecular biology. Results A total of 3600 mosquitoes were collected from 4 cities in Liaoning Province, mainly including Anopheles sinensis (47.2%), Culex pipiens pallens (26.4%), Aedes albopictus (18.1%) and Culex tritaeniorhynchus (6.9%), Wait. The mosquito specimens were divided into 72 groups for grinding, and 2 positive isolates were obtained by tissue culture method. The antigenicity and nucleic acid sequence confirmed that they were type I Japanese encephalitis virus. Conclusion In 2009, Type Ⅰ Japanese encephalitis virus was isolated from Culex pipiens pallens in Anshan area of Liaoning Province and Aedes albopictus in Chaoyang area.