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①目的检测人巨细胞病毒特异性转移因子（ＨＣＭＶ－ＳＴＦ）对小鼠脾细胞分化增殖作用。②方法采用微量细胞培养法培养小鼠脾细胞，共分５组，分别加入１６４０培养液（空白对照），ＰＨＡ，ＨＣＭＶ－Ａｇ，ＨＣＭＶ－ＳＴＦ，ＨＣＭＶ－Ａｇ＋ＨＣＭＶ－ＳＴＦ．培养４８ｈ后加入氚－胸腺嘧啶（３Ｈ－ＴＤＲ），再培养２４ｈ，收集细胞，用液体闪烁计数器计数（ｃｐｍ），计算促进率。③结果ＨＣＭＶ－Ａｇ＋ＨＣＭＶ－ＳＴＦ组与ＨＣＭＶ－Ａｇ，ＨＣＭＶ－ＳＴＦ组比较具有明显的促进小鼠脾细胞分化增殖的作用（ｑ＝３．３５，４．７５，Ｐ＜０．０５）。④结论ＨＣＭＶ－ＳＴＦ具有相应抗原的依赖活性；同时也证明３Ｈ－ＴＤＲ掺入法检测小鼠脾细胞分化增殖效应，是特异性转移因子质控的一种较为理想的测试方法。
Objective To detect the effect of human cytomegalovirus-specific transfer factor (HCMV-STF) on the proliferation and differentiation of mouse spleen cells. MethodsMice spleen cells were cultured by micro-cell culture and divided into 5 groups. The cells were cultured in 1640 medium (PHA, HCMV-Ag, HCMV-STF and HCMV-Ag + HCMV-STF respectively). Tritiated-thymidine (3H-TDR) was added after 48 h of cultivation, and cultured for a further 24 h. Cells were harvested and counted by liquid scintillation counter (cpm) to calculate the promotion rate. Results Compared with HCMV-Ag and HCMV-STF groups, HCMV-Ag + HCMV-STF group had a significant effect of promoting the proliferation and differentiation of mouse spleen cells (q = 3.35, 4.75, P <0.05). Conclusion HCMV-STF has antigen-dependent activity. At the same time, it also proved that 3H-TDR incorporation method can detect the differentiation and proliferation of mouse spleen cells, which is an ideal test method for the quality control of specific transfer factor.