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用MS培养基诱导银杏胚产生愈伤组织.分别在MS培养基中加入不同浓度的卡那霉素、潮霉素和杀稻瘟菌素,将银杏愈伤组织转接到含抗菌素的培养基上继续培养11d之后得到以下结果:MS培养基中含潮霉素25mg/L时,银杏愈伤组织变成褐色,停止生长;MS培养基中含同样浓度的杀稻瘟菌素时,银杏愈伤组织变成浅褐色生长变慢;MS培养基中含同样浓度的卡那霉素时,银杏愈伤组织生长良好.银杏细胞对潮霉素的耐受性很弱,可以作为筛选转基因银杏树种的抗生素使用.
Induction of callus by Ginkgo biloba embryos was induced by MS medium.At the same time, different concentration of kanamycin, hygromycin and blasticidin were added into MS medium, and then the ginkgo callus was transferred to medium containing antibiotics On the 11d after continued training to get the following results: MS medium containing hygromycin 25mg / L, the ginkgo callus brown, stop growing; MS medium containing the same concentration of blasticidin, ginkgo more The tissue became light brown and grew slowly; when the same concentration of kanamycin was added to MS medium, the callus of ginkgo grew well.The tolerance of ginkgo to hygromycin was very weak, which could be used as a screening method for screening transgenic Ginkgo biloba Antibiotic use.