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利用体细胞无性系变异产生新的作物品种,是目前育种界正在开拓的新领域。4月13日在浙江省农科院通过的由王恒立、朱至清、罗树中、金子渔、沈守江、胡含、高明尉等著名专家参加的《大麦体细胞突变育种体系的研究和建立》成果鉴定表明,我国大麦离体诱变技术研究已达到国际先进水平.该项研究在广泛研究影响大麦组织培养诸因素的基础上,建立和完善了幼胚愈伤组织再生植株技术,确定了γ射线处理大麦离体辐射诱变的适宜剂量范围,其中幼胚为1—2kR,幼胚愈伤组织为1—1.5kR,并查明它们的诱变效应.首次建立大麦离体辐射诱变方法和程序,成倍地提高无性系变异频率,尤其是矮秆.千粒重性状的变异频率,如 R9109千粒重达到45—46g,比原供体亲本浙皮1号增加7—8g,株高均在80cm 左右。
The use of somatic clonal variation to produce new crop varieties is a new area currently under development in the breeding community. April 13 in Zhejiang Academy of Agricultural Sciences passed by Wang Hengli, Zhu Zhiqing, Luo Shuzhong, gold fishing, Shen Shoujiang, Hu Han, Gao Ming Wei and other famous experts participated in the “barley somatic mutation breeding system research and establishment of” results identified , The research on in vitro mutagenesis of barley in China has reached the international advanced level.Based on extensive research on various factors that affect the tissue culture of barley, the research established and perfected the technology of regenerating immature embryo callus, and determined the γ-ray treatment of barley The optimal dose range for in vitro radiation mutagenesis was 1 ~ 2kR for immature embryos and 1-1.5kR for immature embryos callus, and their mutagenic effects were identified.It was the first time to establish a method and program for mutagenesis of barley in vitro, The frequency of clonal variation was doubled, especially that of dwarf. The variation frequency of 1000-grain weight traits, such as R9109 1000-grain weight reached 45-46g, was 7-8g higher than that of the original donor Zhepei 1, and the plant height was about 80cm.