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A new indirect inhibitive immunoassay using surface plasmon resonance(SPR) coupled with molecularly imprinted polymer(MIP) was developed.A sulfamethoxazole(SMX) MIP coating capillary was produced and used as an in-tube solid phase extraction(SPE) device.The MIP coating formed a nanometer film on the inner wall of the capillary.The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner.The calibration curve was generated by linear fit over the range of 0.04-10.00 ng/mL.The limit of detection was 0.01 ng/mL.This method has high sensitivity and can be performed automatically.
A new indirect inhibitive immunoassay using surface plasmon resonance (SPR) coupled with molecularly imprinted polymer (MIP) was developed. A sulfamethoxazole (SMX) MIP coating capillary was produced and used as an in-tube solid phase extraction coating formed a nanometer film on the inner wall of the capillary.The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner. The calibration curve was generated by a linear fit over the range of 0.04- 10.00 ng / mL. The limit of detection was 0.01 ng / mL. This method has high sensitivity and can be performed automatically.