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目的研究杏香兔耳风提取物的小肠吸收机制,并初步考察小肠上皮细胞一元羧酸转运蛋白对提取物吸收的影响。方法采用外翻肠囊模型,以杏香兔耳风提取物中绿原酸和3,5-二咖啡酰基奎宁酸为主要成分考察总酚酸在不同肠段(空肠、回肠)中的膜通透性,同时通过吸收抑制(阿魏酸、苯甲酸、布洛芬)试验,考察了一元羧酸转运蛋白对绿原酸和3,5-二咖啡酰基奎宁酸吸收的影响。结果在空肠和回肠中,绿原酸比3,5-二咖啡酰基奎宁酸的小肠渗透率都要高。三种抑制剂(阿魏酸、苯甲酸、布洛芬)可使绿原酸和3,5-二咖啡酰基奎宁酸在回肠的渗透率降低;对绿原酸和3,5-二咖啡酰基奎宁酸在空肠的渗透率影响较小。结论绿原酸和3,5-二咖啡酰基奎宁酸在小肠的吸收属于一级动力学过程,吸收机制为被动扩散。但是同时还存在以一元羧酸转运蛋白介导的主动转运。
OBJECTIVE: To study the intestinal absorption mechanism of the extract from the apricot hornbills ear and to investigate the effect of the monocarboxylate transporter of intestinal epithelial cells on the absorption of the extract. Methods The everted gut capsule model was used to investigate the effects of chlorogenic acid and 3,5-disofacylquinic acid in the extract of Apricot sibiricus rabbits on the membrane permeability of total phenolic acids in jejunum and ileum Permeability, and absorption inhibition (ferulic acid, benzoic acid, ibuprofen) test to examine the monocarboxylic acid transporter on chlorogenic acid and 3,5-dicaffeoylquinic acid absorption. Results In the jejunum and ileum, chlorogenic acid had a higher intestinal permeability than 3,5-disofacylquinic acid. The three inhibitors (ferulic acid, benzoic acid, ibuprofen) decreased the permeability of chlorogenic acid and 3,5-disofoylquinic acid in the ileum. Chlorogenic acid and 3,5-bis Acyl quinic acid in jejunal permeability less affected. Conclusion The absorption of chlorogenic acid and 3,5-dicaffeoylquinic acid in the small intestine belongs to the first-order kinetic process, and the absorption mechanism is passive diffusion. However, there are also active transport mediated by monocarboxylic acid transporters.