论文部分内容阅读
目的研究不同剂量氨基酮戊酸(5-ALA)光动力对人宫颈癌Hela细胞的杀伤作用,为宫颈癌的治疗提供有价值的参考。方法对人宫颈癌细胞株Hela培养、传代后分为两组,观察组给予不同剂量5-ALA光动力,对照组不给予5-ALA光动力,比较两组细胞增殖抑制率、Her-2/neu表达、凋亡率情况。结果观察组早期凋亡率、晚期凋亡率、总凋亡率均高于对照组(P<0.05)。随着5-ALA光敏剂浓度增加,早期凋亡率、晚期凋亡率、总凋亡率也随之增加(P<0.05)。观察组Her-2/neu阳性表达率为12.5%,明显低于对照组(P<0.05)。光动力能量密度5、10、20 J/cm2细胞增殖抑制率明显大于1 J/cm2;10、20 J/cm2细胞增殖抑制率明显大于5 J/cm2;光敏剂浓度1、4 mmol/L细胞增殖抑制率明显大于0.1 mmol/L(P<0.05)。光动力能量密度10、20 J/cm2细胞增殖抑制率比较,差异无统计学意义(P>0.05);光敏剂浓度1、4 mmol/L细胞增殖抑制率比较,差异无统计学意义(P>0.05)。结论 5-ALA光动力对人宫颈癌Hela细胞具有明显的抑制作用,还可诱导细胞凋亡,5-ALA浓度为1 mmol/L,光动力能量密度10 J/cm2是最佳杀伤剂量。
Objective To study the killing effect of photodynamic therapy with different doses of aminolevulinic acid (5-ALA) on human cervical cancer Hela cells and to provide a valuable reference for the treatment of cervical cancer. Methods The human cervical cancer cell line Hela was cultured and divided into two groups. The observation group received 5-ALA photodynamic therapy at different dosages, while the control group did not receive 5-ALA photodynamic therapy. The inhibition rate of Her-2 / neu expression, apoptosis rate. Results The early apoptosis rate, late apoptosis rate and total apoptosis rate in observation group were higher than those in control group (P <0.05). With the increase of 5-ALA photosensitizer concentration, the early apoptosis rate, late apoptosis rate and total apoptosis rate also increased (P <0.05). The positive rate of Her-2 / neu in the observation group was 12.5%, which was significantly lower than that in the control group (P <0.05). Photodynamic power density 5,10,20 J / cm2 inhibition of cell proliferation was significantly greater than 1 J / cm2; 10,20 J / cm2 inhibition of cell proliferation was significantly greater than 5 J / cm2; photosensitizer concentration of 1,4 mmol / L cells Proliferation inhibition rate was significantly greater than 0.1 mmol / L (P <0.05). There was no significant difference in the cell proliferation inhibition rate between 10 and 20 J / cm2 in photodynamic power density (P> 0.05). There was no significant difference in the inhibitory rate of cell proliferation between photosensitizer at 1 and 4 mmol / L (P> 0.05). Conclusions 5-ALA photodynamic therapy can significantly inhibit human cervical cancer Hela cells and induce apoptosis. The best 5-ALA concentration is 1 mmol / L and the photodynamic energy density is 10 J / cm2.