论文部分内容阅读
BLM基因的不同突变是导致Bloom综合征(Bloom’ssyndrome)的病因。BLM基因突变使姐妹染色单体互换率(sisterchromaticexchange,SCE)增高,细胞DNA修复功能降低,这样受损的DNA很容易随细胞分裂而传入子细胞,细胞稳定性下降。临床表现为免疫缺陷,恶性肿瘤易患体质等等。本研究对BLM基因在肿瘤细胞株和正常人中表达的差异进行研究,以期发现肿瘤新的发生机制,为寻找新的特异分子治疗靶点提供线索。应用PTPCR方法检测正常人造血细胞和六种肿瘤细胞株中BLM基因的mRNA水平,并作序列检测。结果表明,六种肿瘤细胞株均高表达BLMmRNA,但未见BLM基因结构异常,而正常人表达量很低(P<0.01)。结论:在本研究中的6种肿瘤细胞株高表达BLMmRNA,肿瘤细胞株和正常人BLMmRNA表达水平有显著差异性。BLM异常可能参与肿瘤的发生或是导致耐药的原因之一。
Different mutations in the BLM gene are the causes of Bloom syndrome. Mutations of BLM gene increase the sister chromatid exchange rate (SCE) and reduce DNA repair function. Thus, damaged DNA can easily be introduced into daughter cells with cell division and cell stability decreases. Clinical manifestations of immune deficiency, susceptibility to malignant tumors and so on. In this study, BLM gene expression in tumor cell lines and normal people to study differences in order to find a new mechanism of tumor, search for new therapeutic targets for specific molecules to provide clues. The PTPCR method was used to detect the mRNA level of BLM gene in normal human hematopoietic cells and six tumor cell lines, and the sequence was detected. The results showed that BLM mRNA was highly expressed in all the six tumor cell lines, but no structural abnormalities in BLM gene were observed, while the expression of BLM mRNA in normal human was very low (P <0.01). CONCLUSIONS: BLM mRNAs are highly expressed in six tumor cell lines in this study, and the expression levels of BLM mRNA in tumor cell lines and normal individuals are significantly different. BLM abnormalities may be involved in the development of cancer or cause resistance one of the reasons.