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目的探讨结肠癌转移相关因子1(MACC1)和肝细胞生长因子(HGF)的表达与卵巢恶性上皮性肿瘤临床、病理特征的关系。方法免疫组织化学法和逆转录-聚合酶链反应(RT-PCR)检测47例上皮性卵巢癌、25例良性卵巢上皮性肿瘤、20例正常卵巢上皮性组织中MACC1、HGF蛋白及二者mRNA的表达。结果 (1)卵巢上皮性癌组织中MACC1和HGF蛋白的阳性表达率及相对表达量均显著高于良性上皮性肿瘤和正常卵巢组织(P均<0.05),而在卵巢良性肿瘤组织与正常卵巢组织中的表达差异无统计学意义(P>0.05);(2)卵巢上皮性癌组织中MACC1及HGF蛋白的表达与患者年龄、组织学类型无关(P均>0.05),与临床分期、组织学分级及淋巴结转移有关(P均<0.05);(3)MACC1 mRNA、HGF mRNA在卵巢上皮性癌组织中的表达量显著高于良性卵巢上皮性肿瘤和正常卵巢组织(F=295.032,P=0.00;F=177.252,P=0.00),且卵巢上皮性癌组织中MACC1 mRNA、HGF mRNA的表达水平呈正相关关系(r=0.810,P<0.01)。结论 MACC1可能在卵巢癌的恶性进展中发挥重要作用,有望成为上皮性卵巢癌诊断和治疗的靶点,与HGF联合检测对判断卵巢上皮性肿瘤预后有重要意义。
Objective To investigate the relationship between the expression of metastasis related factor 1 (MACC1) and hepatocyte growth factor (HGF) in colon and ovarian cancer and the clinical and pathological features. Immunohistochemistry and reverse transcription - polymerase chain reaction (RT-PCR) detected 47 cases of epithelial ovarian cancer, 25 cases of benign epithelial ovarian cancer, 20 cases of normal ovarian epithelial tissue MACC1, HGF both mRNA and protein expression. Results (1) The positive expression rate and relative expression of MACC1 and HGF protein in epithelial ovarian cancer tissues were significantly higher than those in benign epithelial tumors and normal ovarian tissues (all P <0.05), but not in normal ovarian tissues no difference in expression tissue statistically significant (P> 0.05); (2) ovarian epithelial carcinoma MACC1 and HGF protein expression with age, histological type (P> 0.05), clinical stage, histological histological grade and lymph node metastasis (P all <0.05); (3) MACC1 mRNA, expression of HGF mRNA in epithelial ovarian carcinoma is significantly higher than benign epithelial ovarian tumor and normal ovarian tissues (F = 295.032, P = 0.00; F = 177.252, P = 0.00). There was a positive correlation between the expression of MACC1 mRNA and HGF mRNA in ovarian epithelial carcinoma (r = 0.810, P <0.01). Conclusions MACC1 may play an important role in the malignant progression of ovarian cancer and is expected to be the target of diagnosis and treatment of epithelial ovarian cancer. Combined detection with HGF is of great significance in judging the prognosis of epithelial ovarian cancer.