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以 (K冀 5 4 18A∥ 9112 89/LK783)三交F1分离群体的极端不育株和极端可育株分别建立保持池和恢复池 ,利用 79对SSR引物对两池间的多态性进行了研究。分析表明 ,6对SSR引物在两池间扩增出了稳定的多态性差异 ,在分离群体上验证结果表明 ,LK783的育性恢复基因与 4个SSR引物的扩增位点Xgwm11、Xgwm18、Xgwm2 6 4a和Xgwm 2 73有连锁关系 ,该育性恢复基因与Xgwm11、Xgwm18和Xgwm2 73的遗传距离为 6 .5 4± 4 .37cM ,与Xg wm2 6 4a的遗传距离为 5 .71± 4 .10cM ,这 4个引物可应用于K型小麦细胞质雄性不育系育性恢复基因的标记辅助选择。利用中国春缺体四体系和双端体系进一步将Xgwm11、Xgwm18、Xgwm2 6 4a和Xgwm2 73定位于 1BS ,说明LK783的育性恢复基因位于 1BS ,但它在 1BS上的相对位置与Rfv1有所不同 ,它们的等位性关系有待于进一步研究
The maintainer and restorer pools were set up for the extremely sterile and extremely fertile plants of the F1 segregating population of three crosses (K-Ji418A // 911289 / LK783). Polymorphisms between the two pools were determined using 79 pairs of SSR primers Study. The analysis showed that six pairs of SSR primers amplified stable polymorphic differences between the two pools. The validation results of the segregation population showed that the loci of XKwm11, Xgwm18, Xgwm2 6 4a and Xgwm 2 73 had a linkage relationship. The genetic distance between fertility restorer gene and Xgwm11, Xgwm18 and Xgwm2 73 was 6.54 ± 4.37cM, and the genetic distance to Xgwm2 6 4a was 5.71 ± 4 .10cM, these four primers can be applied to marker-assisted selection of fertility restorer genes in K-type wheat CMS lines. Xgwm11, Xgwm18, Xgwm2 6 4a and Xgwm2 73 were further mapped to 1BS by using the four and two-terminal systems of Chinese spring isoforms, indicating that the fertility restorer gene of LK783 was located at 1BS but its relative position on 1BS was different from that of Rfv1 , Their allelic relationship needs further study