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目的 :研究雌激素受体 (estrogenreceptor,ER)基因多态性在中国北京地区绝经后的汉族妇女中的分布及其与骨密度的相关性。方法 :采用聚合酶链式反应 限制性片段长度多态性 (PCR restrictionfragmentlengthpoly morphisms ,PCR RFLP)方法研究ER基因的XbaⅠ和PvuⅡ酶切多态性 ,检测骨密度 ,通过方差分析探讨ER基因的多态性分布与骨密度的关系。结果 :ER基因PvuⅡ酶切多态性分布与桡骨松质骨以及桡骨密质骨的骨密度之间不存在相关性 ,而ER基因XbaⅠ酶切多态性分布与桡骨松质骨以及桡骨密质骨的骨密度之间存在相关性 ,XX基因型骨密度值最低 ,xx基因型骨密度值最高。结论 :ER基因XbaⅠ酶切多态性分布与桡骨松质骨以及桡骨密质骨的骨密度之间显著相关 (P <0 .0 5 )。本研究为探讨骨质疏松症在分子生物学范畴的发病机制及预防与预后提供了有益的依据
OBJECTIVE: To study the distribution of estrogen receptor (ER) gene polymorphism and its relationship with bone mineral density in postmenopausal Chinese women in Beijing, China. Methods: PCR restriction fragment length polymorphisms (PCR RFLP) were used to study the polymorphisms of Xba Ⅰ and Pvu Ⅱ in ER gene. The bone mineral density (BMD) was determined by ANOVA and the polymorphism of ER gene Relationship between sexual distribution and bone mineral density. Results: There was no correlation between polymorphism of ER gene Pvu Ⅱ digestion and BMD of radial cancellous bone and radial dense bone. However, the distribution of ER gene Xba Ⅰ polymorphism was associated with radial cancellous bone and radial mass There was a correlation between bone mineral density, XX genotype lowest bone mineral density, xx genotype highest bone mineral density. CONCLUSION: The distribution of Xba Ⅰ polymorphism of ER gene is significantly associated with the BMD of radial and cancellous bone (P <0.05). This study provides a useful basis for exploring the pathogenesis and prevention and prognosis of osteoporosis in molecular biology