目的就中国海域分离的啮氏艾肯菌对小鼠的毒力及伤口感染情况进行研究,并进行抗生素敏感性实验。方法昆明小鼠腹腔注射啮氏艾肯菌悬液,观察一般状态、白细胞计数和血培养。第5天脱颈处死后解剖取脏器甲醛固定,石蜡包埋、制片,HE染色,光镜观察;昆明小鼠致伤后浸泡在含啮氏艾肯菌的人工海水中45～60min,打捞后分笼于层流柜中饲养。于致伤浸泡后不同时间做白细胞计数及血培养,并观察一般状态和伤口炎性反应,定时取伤肢分泌物做细菌培养;实验完毕后伤肢甲醛固定,石蜡包埋、制片,HE染色,光镜检查;用K-B法做啮氏艾肯菌对各种抗生素药物敏感性实验。结果小鼠注射啮氏艾肯菌液后,白细胞升高,感染12h后20%小鼠心和肝脏有细菌生长。小鼠感染啮氏艾肯菌后,病变部位以肺、肝及肾脏为重,病变性质主要是造成血液循环障碍,引起不同程度的充血、出血及血停滞现象。致伤小鼠经含啮氏艾肯菌的海水浸泡后,6、12、24、48、72h伤肢培养均为阳性,感染后48h时20%小鼠肠组织细菌培养阳性,其余时间点和其他组织细菌培养均为阴性。伤肢组织可见肌纤维排列疏松、紊乱、断裂,横纹肌间较多炎细胞浸润。啮氏艾肯菌对复方新诺明、哌拉西林、美洛培南、磺胺的敏感性仅为67%,对其他抗生素均表现为敏感。结论中国海域分离的啮氏艾肯菌具有致病力,啮氏艾肯菌对复方新诺明、哌拉西林、美洛培南、磺胺的敏感性较低,对其他抗生素均表现为敏感。 OBJECTIVE To study the virulence and wound infection of mice infected with Echinochloa crusgalli isolated in China sea area and conduct antibiotic susceptibility testing. Methods Kunming mice were injected intraperitoneally with E.cavarum suspension to observe the general status, white blood cell count and blood culture. On day 5, the organs of the organs were dissected and fixed with paraffin, embedded in paraffin, and were observed by HE staining and light microscopy. Kunming mice were immersed in artificial seawater containing Acridus naevela for 45-60min after injury, Caught after cage cage in the laminar feeder. The leukocyte count and blood culture were taken at different times after wounding and injury, and the general state and inflammatory reaction of the wound were observed. Bacterial culture was taken from the wound excretion regularly. After the experiment, the injured limbs were fixed with formaldehyde, embedded in paraffin, Staining, light microscopy; using KB method to do Eisenia coli antibiotic susceptibility tests. Results After the mice were injected with Ehrlichia coli, the white blood cells were increased. After 12 hours of infection, 20% of the mice had bacterial growth in the heart and liver. After the mice infected with Rickettsia acuminatum, the lesions were mainly lung, liver and kidney, and the pathological changes were mainly caused by blood circulation disorder, causing congestion, bleeding and blood stagnation. Injured mice were immersed in seawater containing E. avermitilis for 6, 12, 24, 48 and 72 hours, and all wound cultures were positive. Twenty (48) hours after infection, 20% Bacterial culture in other tissues were negative. Injured limbs showed muscle fiber arranged loose, disorder, rupture, striated muscle inflammation more infiltration. Echinococcus of Cynanchum fungicide compound cotrimoxazole, piperacillin, meropenem, sulfa sensitivity of only 67%, the performance of other antibiotics are sensitive. Conclusion Acinetobacter baumannii isolated from China has pathogenicity. Anemone has low sensitivity to cotrimoxazole, piperacillin, meropenem and sulfonamides and is sensitive to other antibiotics.