论文部分内容阅读
将23只帕金森病(PD)成功模型大鼠分别进行11C-Raclopride PET显像,ROI方法测量模型大鼠两侧纹状体/小脑比值并进行统计分析,比较两侧有无显著性差异。正常与模型大鼠分别进行TH免疫组化染色。结果表明,模型大鼠毁损侧纹状体放射性明显上升,两侧纹状体/小脑比值统计比较,p值为0.000,具有显著性差异;同时,模型大鼠TH染色黑质处阳性神经元数量减少。通过PET多巴胺D2受体成像在体显示PD模型大鼠毁损侧纹状体D2受体状态,再结合行为学观察可作为证实模型成功的标准之一,为基础研究和临床诊断PD提供了一种分子成像工具。
The rats of 23 Parkinson’s disease (PD) success models were subjected to 11C-Raclopride PET imaging. ROI was used to measure the ratio of striatum to cerebellum on both sides of the model rats and statistical analysis was performed to compare whether there was significant difference between the two sides. Normal and model rats were immunohistochemically stained for TH. The results showed that the radioactivity of the lesioned striatum increased significantly in the model rats, the striatum / cerebellum ratio on both sides statistically compared, p = 0.000, with significant differences; the same time, the number of TH-stained substantia nigra neurons cut back. PET dopamine D2 receptor imaging in vivo showed PD model rats damaged lateral striatum D2 receptor status, combined with behavioral observation can be used as a model to confirm the success of the standard for basic research and clinical diagnosis of PD provides a Molecular imaging tools.