采用高通量454焦磷酸测序方法对宁波沿海2个重点排污口、8个一般排污口的20个站位水样进行分析,得到2011年的3月、5月、8月和10月份各排污口蓝藻和含叶绿体单细胞生物的分布情况。研究结果表明:宁波沿海陆源排污口中含叶绿体单细胞生物数量最多,占91.857%,包括眼虫门(Euglenozoa)、绿藻门(Chlorophyta)、隐藻门(Cryptophyta)生物。其中主要为眼虫门的裸藻纲(Euglenoidea)生物占90.08%。蓝藻门的集球藻目(Synechococcophycideae)和颤藻亚目(Oscillatoriophycideae)含量相对较多,分别占5.70%和1.79%;排污口中裸藻纲生物数量季节性差异比较大,3月份、8月份数量相对较多,5月份和10月份较少,推测这类生物的数量与季节性温度变化有关;各排污口污染物、这两类生物检出量和种类有一定差异,在氮磷含量高的排污口(如S9号)其检出量并不高,推测检出量与排污口的主要污染物中氨氮含量没有直接关系,可能受氮磷比的影响。 High-throughput 454 pyrosequencing method was used to analyze the water samples of 20 stations along the two major pollutant discharge ports and 8 general pollutant discharge ports along the coast of Ningbo, and the pollutants were collected in March, May, August and October of 2011 Distribution of cyanobacteria and chloroplast-containing single-celled organisms. The results showed that the number of chloroplast-containing single-cell organisms in land-sea pollutants from coastal areas in Ningbo was the highest (91.857%), including Euglenozoa, Chlorophyta and Cryptophyta. Among them, Euglenoidea, which is mainly euglena, accounted for 90.08%. Synechococcophycideae and Oscillatoriophycideae in Cyanophyta were relatively high, accounting for 5.70% and 1.79% of the total, respectively. Seasonal differences in the number of Euglena species in the sewage outfall were relatively large. In March and August, Relatively more in May and less in October, speculated that the number of such organisms and seasonal temperature changes; pollutants at the sewage outfall, the two types of biological detection of quantity and type are some differences in high nitrogen and phosphorus content Sewage outfall (such as S9) the detection of the amount is not high, presumably the amount of sewage outfall and the main pollutants in the ammonia content is not directly related, may be affected by the ratio of nitrogen and phosphorus.